Neurofilament Light Chain
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Neurofilament Light Chain (NfL), Serum/Plasma (Test Site EORLA)
Test ID: NFLRO S
Test
Method
Roche Elecsys
Platform
Electrochemiluminescence
Report Includes
Specimens
Serum; Plasma (Na Heparin);
Important Note: TEST ID: NFLRO S
Non-interfaced clients should specify the Test ID TEST ID: NFLRO S when ordering this test. Not doing so will cause processing delays while ICL ascertains which test you are requesting.
Similar testing is available for clinical use at St. Michael’s Hospital: Neurofilament Light Chain (NfL), Serum/Plasm
Important Note: TEST ID: NFLRO S
Non-interfaced clients should specify the Test ID TEST ID: NFLRO S when ordering this test. Not doing so will cause processing delays while ICL ascertains which test you are requesting.
Similar testing is available for clinical use at St. Michael’s Hospital: Neurofilament Light Chain (NfL), Serum/Plasm
Clinical Utility
Multiple sclerosis, other neurodegenerative disorders, traumatic brain injury.
Neurofilament Light Chain (NfL) is a biomarker of neuraxonal damage and disease activity and is associated with clinical and MRI outcomes in Multiple Sclerosis patients. High or increasing values are suggestive of increased neurodegeneration and increased risk of progression. Normalized or decreasing values are suggestive of a better prognosis and/or effective treatment.
Reference:
M.S. Freedman, S. Gnanapavan, R.A. Booth, P.A. Calabresi, M. Khalil, J. Kuhle, J. Lycke, T. Olsson, C. et al., Guidance for use of neurofilament light chain as a cerebrospinal fluid and blood biomarker in multiple sclerosis management, eBioMedicine 101 (2024) 104970. https://doi.org/10.1016/j.ebiom.2024.104970.
Test Location
The Ottawa Hospital General Campus, 501 Smyth Road, Ottawa ON K1H 8L6
Test Version
13-Mar-2025
Specimen
Specimens
Serum; Plasma (Na Heparin);
Important Note: TEST ID: NFLRO S
Non-interfaced clients should specify the Test ID TEST ID: NFLRO S when ordering this test. Not doing so will cause processing delays while ICL ascertains which test you are requesting.
Similar testing is available for clinical use at St. Michael’s Hospital: Neurofilament Light Chain (NfL), Serum/Plasm
Important Note: TEST ID: NFLRO S
Non-interfaced clients should specify the Test ID TEST ID: NFLRO S when ordering this test. Not doing so will cause processing delays while ICL ascertains which test you are requesting.
Similar testing is available for clinical use at St. Michael’s Hospital: Neurofilament Light Chain (NfL), Serum/Plasm
Collection Containers
Preferred
Gold top (clot activator & gel)
Acceptable
Red top (no additive); Na Heparin (Green Top)
Sample Volume
2.0 mL
Minimum Volume
0.5 mL
Pediatric Volume
1.0 mL
Collection & Handling
Handling Information
Store and send frozen.
Stability
| Ambient | 48 hours |
|---|---|
| Refrigerated | 7 days |
| Frozen | >7 days |
Additional Stability Information
Stable for up to 4 freeze-thaw cycles.
Test Version
13-Mar-2025
Performance / Interpretation
Method
Roche Elecsys
Platform
Electrochemiluminescence
Turnaround Time
16 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Neurofilament Light Chain | pg/mL | |||
|
Analysis performed by Roche Elecsys. Note, values are significantly (~85%) lower than Simoa. NfL is a biomarker of neuroaxonal damage and disease activity and is associated with clinical and MRI outcomes in Multiple Sclerosis patients. High or increasing values are suggestive of increased neurodegeneration and increased risk of progression. Normalized or decreasing values are suggestive of a better prognosis and/or effective treatment. A change in NfL values >30% is beyond the analytical variation and may indicate clinically relevant changes in NfL results when interpreted in conjunction with clinical findings. This test has been validated for clinical use. To convert previous Simoa NfL values to Elecsys values please use the following regression equation: NfL(Elecsys) = 0.1227*NfL(Simoa) + 0.1862 Reference intervals (97.5th percentile) determined using BMI = 27.2 kg/m^2, adapted from Benkert et al. (The Lancet Neurology. 21 (2022) 246–257) |
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Test Location
The Ottawa Hospital General Campus, 501 Smyth Road, Ottawa ON K1H 8L6
Test Version
13-Mar-2025
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| NFLRO S | 64244Neurofilament Light Chain | pg/mL |
Test Version
13-Mar-2025