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Comprehensive Autoimmune Encephalitis Panel (LHSC) , CSF

Test ID: AEP CSF

Test

Aliases/Synonyms

Paraneoplastic antibodies

Method

Various

Method Description

Cell-based assay (CBA) and tissue indirect immunofluorescence (TIIF) assay for autoimmune encephalitis antibodies (reported based on CBA).
Immunoblot and tissue indirect immunofluorescence (TIIF) assay for paraneoplastic antibodies (reported based on immunoblot).
ELISA and immunoblot for Anti-GAD65.
Each sample will also be screened for neural antibodies based on research use-only TIIF, only negative reported on a research basis.

Report Includes

Autoimmune Encephalitis Antibodies:; Anti-NMDAR; Anti-LGI1; Anti-CASPR2; Anti-AMPAR1/R2; Anti-GABARB1/B2; Anti-DPPX
Paraneoplastic Antibodies:Anti-Hu (ANNA-1); Anti-Ri (ANNA-2); Anti-Yo (PCA-1); Anti-Amphiphysin; Anti-CV2 (CRMP5); Anti-Ma2/Ta (PNMA2); Anti-Tr (DNER); Anti-Zic4; Anti-Recoverin; Anti-Titin; Anti-SOX1;
Other Antibody: Anti-GAD65
Research-only Neural Antibodies:; Anti-GFAP; Anti-mGluR1; Anti-NIF; Anti-Neurochondrin; ANNA-3; PCA-2/Anti-MAP1B; Anti-AP3B2; Anti-PDE10A; Anti-KLHL11; Anti-ITPR1; Anti-GRAF

Specimens

CSF

Submission of a concurrent serum is recommended (separate billing): Comprehensive Autoimmune Encephalitis Panel (LHSC) , Serum

Submission of a completed Autoimmune Encephalitis Panel Questionnaire is recommended to facilitate laboratory interpretation and prompt clinical-serological correlation by a laboratory scientist with the ordering physician.

Paraneoplastic Antibodies included in this Panel may be ordered on their own: Paraneoplastic Antibodies Expanded Panel (LHSC), CSF

Clinical Utility

A clinical diagnosis should not be made solely based on a neural antibody result; results should be interpreted in the context of the patients clinical presentation and other ancillary test findings to ensure accurate diagnosis.

Test Location

London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5

Test Version

11-Nov-2022

Specimen

Specimens

CSF

Submission of a concurrent serum is recommended (separate billing): Comprehensive Autoimmune Encephalitis Panel (LHSC) , Serum

Submission of a completed Autoimmune Encephalitis Panel Questionnaire is recommended to facilitate laboratory interpretation and prompt clinical-serological correlation by a laboratory scientist with the ordering physician.

Paraneoplastic Antibodies included in this Panel may be ordered on their own: Paraneoplastic Antibodies Expanded Panel (LHSC), CSF

Sample Volume

1 mL

Collection & Handling

Handling Information

Store and send cold or frozen.

Stability

Refrigerated 14 days
Frozen >14 days

Test Version

11-Nov-2022

Performance / Interpretation

Method

Various

Method Description

Cell-based assay (CBA) and tissue indirect immunofluorescence (TIIF) assay for autoimmune encephalitis antibodies (reported based on CBA).
Immunoblot and tissue indirect immunofluorescence (TIIF) assay for paraneoplastic antibodies (reported based on immunoblot).
ELISA and immunoblot for Anti-GAD65.
Each sample will also be screened for neural antibodies based on research use-only TIIF, only negative reported on a research basis.

Turnaround Time

10 days

Results

Name Units Reference Range Conversion Factor
Various
  • Negative
Anti-GAD65 ELISA IU/mL
  • ≤ 5

Test Location

London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5

Test Version

11-Nov-2022

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
AEP CSF 63469Anti-AMPAR1/R2 CBA,CSF
63470Anti-CASPR2 CBA,CSF
63471Anti-DPPX CBA,CSF
63472Anti-GABARB1/B2 CBA,CSF
63473Anti-LGI1 CBA,CSF
63474Anti-NMDAR CBA,CSF
63475Anti-Amphiphysin Immunoblot, CSF
63476Anti-CV2 Immunoblot, CSF
63477Anti-GAD65 Immunoblot, CSF
63478Anti-Hu Immunoblot, CSF
63479Anti-Ma2:Ta Immunoblot, CSF
63480Anti-SOX1 Immunoblot, CSF
63481Anti-Recoverin Immunoblot, CSF
63482Anti-Ri Immunoblot, CSF
63483Anti-Titin Immunoblot, CSF
63484Anti-Tr (DNER) Immunoblot, CSF
63485Anti-Yo Immunoblot, CSF
63486Anti-Zic4 Immunoblot, CSF
63488Antibody Result, CSF
63489Research Based Antibody Screen,TIIF,CSF

Test Version

11-Nov-2022