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Comprehensive Autoimmune Encephalitis Panel (LHSC) , Serum

Test ID: AEP SP

Test

Aliases/Synonyms

Paraneoplastic antibodies

Method

Various

Method Description

Cell-based assay (CBA) and tissue indirect immunofluorescence (TIIF) assay for autoimmune encephalitis antibodies (reported based on CBA).
Immunoblot and tissue indirect immunofluorescence (TIIF) assay for paraneoplastic antibodies (reported based on immunoblot).
ELISA and immunoblot for Anti-GAD65.
Each sample will also be screened for neural antibodies based on research use-only TIIF, only negative reported on a research basis.

Report Includes

Autoimmune Encephalitis Antibodies:; Anti-NMDAR; Anti-LGI1; Anti-CASPR2; Anti-AMPAR1/R2; Anti-GABARB1/B2; Anti-DPPX
Paraneoplastic Antibodies:Anti-Hu (ANNA-1); Anti-Ri (ANNA-2); Anti-Yo (PCA-1); Anti-Amphiphysin; Anti-CV2 (CRMP5); Anti-Ma2/Ta (PNMA2); Anti-Tr (DNER); Anti-Zic4; Anti-Recoverin; Anti-Titin; Anti-SOX1;
Other Antibody: Anti-GAD65
Research-only Neural Antibodies:; Anti-GFAP; Anti-mGluR1; Anti-NIF; Anti-Neurochondrin; ANNA-3; PCA-2/Anti-MAP1B; Anti-AP3B2; Anti-PDE10A; Anti-KLHL11; Anti-ITPR1; Anti-GRAF

Specimens

Serum

Submission of a completed Autoimmune Encephalitis Panel Questionnaire is recommended to facilitate laboratory interpretation and prompt clinical-serological correlation by a laboratory scientist with the ordering physician.

Paraneoplastic Antibodies included in this Panel may be ordered on their own: Paraneoplastic Antibodies Expanded Panel (LHSC), Serum/Plasma

Clinical Utility

A clinical diagnosis should not be made solely based on a neural antibody result; results should be interpreted in the context of the patients clinical presentation and other ancillary test findings to ensure accurate diagnosis.

Test Location

London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5

Test Version

11-Nov-2022

Specimen

Specimens

Serum

Submission of a completed Autoimmune Encephalitis Panel Questionnaire is recommended to facilitate laboratory interpretation and prompt clinical-serological correlation by a laboratory scientist with the ordering physician.

Paraneoplastic Antibodies included in this Panel may be ordered on their own: Paraneoplastic Antibodies Expanded Panel (LHSC), Serum/Plasma

Collection Containers

Gold top (SST); Red top (no additive)

Sample Volume

1 mL

Collection & Handling

Handling Information

Store and send cold or frozen.

Stability

Refrigerated 14 days
Frozen >14 days

Test Version

11-Nov-2022

Performance / Interpretation

Method

Various

Method Description

Cell-based assay (CBA) and tissue indirect immunofluorescence (TIIF) assay for autoimmune encephalitis antibodies (reported based on CBA).
Immunoblot and tissue indirect immunofluorescence (TIIF) assay for paraneoplastic antibodies (reported based on immunoblot).
ELISA and immunoblot for Anti-GAD65.
Each sample will also be screened for neural antibodies based on research use-only TIIF, only negative reported on a research basis.

Turnaround Time

10 days

Results

Name Units Reference Range Conversion Factor
Various
  • Negative
Anti-GAD65 ELISA IU/mL
  • ≤ 5

Test Location

London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5

Test Version

11-Nov-2022

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
AEP SP 63490Anti-AMPAR1/R2 CBA,Serum
63491Anti-CASPR2 CBA,Serum
63492Anti-DPPX CBA,Serum
63493Anti-GABARB1/B2 CBA,Serum
63494Anti-LGI1 CBA,Serum
63495Anti-NMDAR CBA,Serum
63496Anti-Amphiphysin Immunoblot,Serum
63497Anti-CV2 Immunoblot,Serum
63498Anti-GAD65 Immunoblot,Serum
63499Anti-Hu Immunoblot,Serum
63500Anti-Ma2:Ta Immunoblot,Serum
63501Anti-SOX1 Immunoblot,Serum
63502Anti-Recoverin Immunoblot,Serum
63503Anti-Ri Immunoblot,Serum
63504Anti-Titin Immunoblot,Serum
63505Anti-Tr (DNER) Immunoblot,Serum
63506Anti-Yo Immunoblot,Serum
63507Anti-Zic4 Immunoblot,Serum
63508Anti-GAD65 Elisa,Serum
63509Antibody Result,Serum
63510Research Based Antibody Scr.,TIIF,Serum

Test Version

11-Nov-2022