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Hemoglobin Fractionation, Blood

Test ID: HBELEC
Test
Specimen
Performance / Interpretation
Interface / Setup

Test

Aliases/Synonyms

Hb Fractionation; Hemoglobin Electrophoresis; Hemoglobinopathy Investigation

Method

Capillary Electrophoresis

Method Description

Includes hemoglobin electrophoresis gel card and HbH preparation if necessary based on diagnostic criteria.

Platform

Sebia Capillarys 2 Flex

Report Includes

Hemoglobin A2; Hemoglobin F; Hemoglobin A; Sickle Cell Screen; Hemoglobin Variants; Interpretation

Specimens

Whole Blood

Clinical Utility

This test should be ordered if a hemoglobinopathy or thalassemia are being considered. Test is performed by HPLC (High Performance Liquid Chromatography) which results in a separation of hemoglobin subtypes and an accurate assessment of Hb A2 (for B thal diagnosis). Also included, if appropriate, is a Hb H preparation to exclude alpha thalassemia. A CBC and ferritin result should be included to assist in the interpretation of results. (Bah BJ, Hemoglobinopathy Diagnosis. Blackwell Science, United Kindom, 2001:20)

Test Version

15-Sep-2025

Specimen

Specimens

Whole Blood

Collection Containers

Lavender top (EDTA); Yellow top (ACD)

Sample Volume

4.0 mL

Minimum Volume

3.0 mL

Collection & Handling

Handling Information

Frozen specimens are unacceptable – do not freeze. Store and send cold.

Additional Information

You must provide Hemoglobin, MCV, RDW and date of birth. This information may not be included in the report but is required for interpretation of findings (included in report). Hemoglobin H (HbH) screen may not be done unless MCV is less than 75. Hemolyzed specimens may yield uninterpretable results.

Stability

Refrigerated 7 days
Frozen Unacceptable

Rejection Criteria

Specimen Frozen

Test Version

15-Sep-2025

Performance / Interpretation

Method

Capillary Electrophoresis

Method Description

Includes hemoglobin electrophoresis gel card and HbH preparation if necessary based on diagnostic criteria.

Platform

Sebia Capillarys 2 Flex

Turnaround Time

9 days

Results

Name Units Reference Range Conversion Factor
Hemoglobin A %
  • Up to 1 d: 8.2 - 28.5
  • 2 - 28 d: 3.7 - 45.4
  • 29 - 60 d: 11.8 - 81.0
  • 61 d - 4 m: 35.6 - 99.9
  • 4 - 6 m: 85.9 - 99.9
  • 6 - 12 m: 95.9 - 99.9
  • 12 m to Adult: 96.5 - 100.0
Hemoglobin F %
  • Up to 1 d: 71.5 - 91.8
  • 2 - 28 d: 54.6 - 96.3
  • 29 - 60 d: 19.0 - 88.2
  • 61 d - 4 m: 0.1 - 64.4
  • 4 - 6 m: 0.1 - 14.1
  • 6 - 12 m: 0.1 - 4.1
  • 12 m to Adult: <1.0
Hemoglobin A2 %
  • 2.0 - 3.5

Test Version

15-Sep-2025

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
HBELEC 8698Hb A %
8697Hb F %
8696Hb A2 %
8694Sickle Cell Screen
8695Hb H Preparation
63543Cellulose Acetate
40002Tech Comment
65163Hemoglobin (G/L) in blood g/L
65164MCV (FL) fL
65165RDW-CV %
65166HBS %
65167HBC %
65168HBE %
65169HB LEPORE %
65170HBD %
65171HBH %
65172HBG %
65173HBJ %
65900 Unidentified Hb %
38470Hematologist Interpretation

Test Version

15-Sep-2025