Back to Test Catalogue

17-Hydroxypregnenolone, Serum

Test ID: 17OHPL
Test
Specimen
Performance / Interpretation
Interface / Setup

Test

Aliases/Synonyms

17 Hydroxypregnenolone; 17OH-Pregnenolone

Method

Chromatography/Mass Spectrometry

Report Includes

17 Hydroxypregnenolone

Specimens

Serum

Clinical Utility

17-Hydroxypregnenolone is useful in the diagnosis of 3-Beta-Hydroxylase enzyme deficiency, a rare cause of congenital adrenal hyperplasia, and 17-Hydroxylase (P450c17) enzyme deficiency.

Test Version

19-Nov-2025

Specimen

Specimens

Serum

Collection Containers

Red top (no additive)

Sample Volume

2.0 mL

Minimum Volume

0.4 mL

Collection & Handling

Handling Information

Store and send frozen.

Stability

Ambient 24 hours
Refrigerated 5 days
Frozen 28 days

Rejection Criteria

Hemolysis Gross
Lipemic Gross
Icteric Gross
Specimen Gel separator tube

Test Version

19-Nov-2025

Performance / Interpretation

Method

Chromatography/Mass Spectrometry

Turnaround Time

11 days

Results

Name Units Reference Range Conversion Factor
17 Hydroxypregnenolone ng/dL
  • Adult Males: ≤ 700
  • Adult Females, Pre-Menopausal:
    Mid Follicular: 38 - 378
    Surge: 33 - 583
    Mid Luteal: 70 - 470
  • Pediatric Male and Female::
  • < 30 days: ≤ 3013
  • 1-11 months: ≤ 869
  • 1 year: ≤ 557
  • 2 years: ≤ 487
  • 3 years: ≤ 541
  • 4 years: ≤ 561
  • 5 years: ≤ 564
  • 6 years: ≤ 561
  • 7 years: ≤ 549
  • 8 years: ≤ 569
  • 9 years: ≤ 590
  • 10 years: ≤ 594
  • 11 years: ≤ 629
  • 12 years: ≤ 666
  • 13 years: ≤ 678
  • 14 years: ≤ 706
  • 15 years: ≤ 735
  • 16 years: ≤ 739
  • 17 years: ≤ 756

Test Version

19-Nov-2025

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
17OHPL 6205117 HYDROXYPREGNENOLONE ng/dL

Test Version

19-Nov-2025