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Extractable Nuclear Antigen (ENA) Antibodies, Serum

Test ID: ENAAB
Orderable by Ontario ND

Test

Aliases/Synonyms

ANF; Anti-nuclear antibody; Nuclear Antibodies; ANA

Method

Multiplex Immunoassay

Report Includes

ANA/ENA Screen; dsDNA Antibodies; Chromatin Antibodies; Ribosomal Protein Antibodies; SS-A 52 Antibodies; SS-A 60 Antibodies; SS-B Antibodies; Centromere-B Antibodies; Sm Antibodies; SmRNP Antibodies; RNP 68 Antibodies; RNP A Antibodies; Scl-70 Antibodies; Jo-1 Antibodies

Specimens

Serum

Clinical Utility

Specimen will be tested for antibodies to 13 nuclear antigens and the results compared to pre-established cutoffs for systemic autoimmune disease. Numeric results for the antibodies will only be reported when one antibody or more exceed the cutoff. Every report will include an interpretive Medical Decision-Support System (MDSS) comment.

Test Location

Laboratory Reference Centre, 237 Barton Street E, Hamilton ON L8L 2X2

Test Version

4-Oct-2023

Specimen

Specimens

Serum

Sample Volume

1 mL

Stability

Refrigerated 7 days
Frozen > 7 days

Test Version

4-Oct-2023

Performance / Interpretation

Method

Multiplex Immunoassay

Turnaround Time

5 days

Results

Name Units Reference Range Conversion Factor
ANA/ENA Screen
  • Negative
dsDNA Antibodies IU/mL
  • Negative: ≤ 4
  • Indeterminate: 5 - 9
  • Positive: ≥ 10
Chromatin Antibodies AI
  • <1.0
Ribosomal Protein Antibodies AI
  • <1.0
SS-A 52 Antibodies AI
  • <1.0
SS-A 60 Antibodies AI
  • <1.0
SS-B Antibodies AI
  • <1.0
Centromere-B Antibodies AI
  • <1.0
Sm Antibodies AI
  • <1.0
SmRNP Antibodies AI
  • <1.0
RNP 68 Antibodies AI
  • <1.0
RNP A Antibodies AI
  • <1.0
Scl-70 Antibodies AI
  • <1.0
Jo-1 Antibodies AI
  • <1.0

Test Location

Laboratory Reference Centre, 237 Barton Street E, Hamilton ON L8L 2X2

Test Version

4-Oct-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
ENAAB 29570ANA Screen
Note:HL7 codes (Effective September 11, 2023)

Test Version

4-Oct-2023