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5-Hydroxyindole Acetic Acid (5-HIAA), 24 hr Urine

Test ID: 5HIAA
Orderable by Ontario ND

Test

Aliases/Synonyms

5HIAA

Method

LC-MS/MS

Report Includes

Creatinine; 5-Hydroxyindole Acetic Acid (µmol/L); 5-Hydroxyindole Acetic Acid (µmol/d)

Specimens

Urine (24 hr)

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

16-Nov-2022

Specimen

Specimens

Urine (24 hr)

Collection Containers

Urine Container (24hr) 4L

Sample Volume

10 mL

Patient Preparation

The following foods and medications should be avoided for three (3) days prior to the collection of urine, and during the collection of the sample:
Foods containing high hydroxyindole concentrations (e.g. aubergines, avocados, bananas, black- and red-currants, kiwi, gooseberries, melons, tomatoes, plums, pineapples and walnuts) as well as Glyceryl Guaiacolate (an ingredient in some cough medicines) can increase urinary 5-HIAA excretion. Pregnancy and sleep deprivation can also result in raised 5-HIAA excretion.
Medications and drugs such as Levodopa, Methyldopa, Monoamine Oxidase Inhibitors, Ethanol, Imipramine, Isoniazid, Reserpine and p-Chlorophenylalanine can reduce 5- HIAA production. Others, such as Acetanilide, Phenacetin, Methocarbamol, Cisplatin, Fluorouracil, Melphalan and Rauwolfia Alkaloids can increase urinary 5-HIAA, as can 5- Hydroxytryptophan, which is used to treat depression and is available over-the-counter.

Collection & Handling

Collection Instructions

24-hour urine collected in a polyethylene container with 25 mL 6N Hydrochloric Acid. It is important that the urine is refrigerated during the collection. The pH of the urinemustbe < 4.0.

Handling Information

The final pH must be maintained from 2 – 4.

Additional Information

Provide 24 hour volume and date of collection.

Stability

Refrigerated 1 month
Frozen > 1 month

Rejection Criteria

Specimen pH > 5.0

Test Version

16-Nov-2022

Performance / Interpretation

Method

LC-MS/MS

Turnaround Time

10 days

Results

Name Units Reference Range Conversion Factor
Creatinine mmol/d
  • Male: 9.0 - 17.0
  • Female: 7.0 - 15.0
5-Hydroxyindole Acetic Acid µmol/L
  • Not Available
5-Hydroxyindole Acetic Acid µmol/d
  • 0 - 50

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

16-Nov-2022

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
5HIAA 7601Volume L
7602Creatinine/D 24HIA mmol/d
7637Creatinine/L mmol/L
280125HIAA 24H umol/d
280105HIAA per litre umol/L

Test Version

16-Nov-2022