5HIAA
5-Hydroxyindole Acetic Acid (5-HIAA), 24 hr Urine
Test
Aliases/Synonyms
Method
LC-MS/MS
Report Includes
Creatinine; 5-Hydroxyindole Acetic Acid (µmol/L); 5-Hydroxyindole Acetic Acid (µmol/d)
Specimens
Urine (24 hr)
Test Location
St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8
Test Version
16-Nov-2022
Specimen
Specimens
Urine (24 hr)
Collection Containers
Urine Container (24hr) 4L
Sample Volume
10 mL
Patient Preparation
The following foods and medications should be avoided for three (3) days prior to the collection of urine, and during the collection of the sample:
Foods containing high hydroxyindole concentrations (e.g. aubergines, avocados, bananas, black- and red-currants, kiwi, gooseberries, melons, tomatoes, plums, pineapples and walnuts) as well as Glyceryl Guaiacolate (an ingredient in some cough medicines) can increase urinary 5-HIAA excretion. Pregnancy and sleep deprivation can also result in raised 5-HIAA excretion.
Medications and drugs such as Levodopa, Methyldopa, Monoamine Oxidase Inhibitors, Ethanol, Imipramine, Isoniazid, Reserpine and p-Chlorophenylalanine can reduce 5- HIAA production. Others, such as Acetanilide, Phenacetin, Methocarbamol, Cisplatin, Fluorouracil, Melphalan and Rauwolfia Alkaloids can increase urinary 5-HIAA, as can 5- Hydroxytryptophan, which is used to treat depression and is available over-the-counter.
Collection & Handling
Collection Instructions
24-hour urine collected in a polyethylene container with 25 mL 6N Hydrochloric Acid. It is important that the urine is refrigerated during the collection. The pH of the urinemustbe < 4.0.
Handling Information
The final pH must be maintained from 2 – 4.
Additional Information
Provide 24 hour volume and date of collection.
Stability
Refrigerated | 1 month |
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Frozen | > 1 month |
Rejection Criteria
Specimen | pH > 5.0 |
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Test Version
16-Nov-2022
Performance / Interpretation
Method
LC-MS/MS
Turnaround Time
10 days
Results
Name | Units | Reference Range | Conversion Factor | |
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Creatinine | mmol/d |
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5-Hydroxyindole Acetic Acid | µmol/L |
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5-Hydroxyindole Acetic Acid | µmol/d |
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Test Location
St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8
Test Version
16-Nov-2022
Interface / Setup
HL7 Interface Codes
Order Code | Result Codes | Units |
---|---|---|
5HIAA | 7601Volume | L |
7602Creatinine/D 24HIA | mmol/d | |
7637Creatinine/L | mmol/L | |
280125HIAA 24H | umol/d | |
280105HIAA per litre | umol/L |
Test Version
16-Nov-2022