Test Overview
Clinical Utility
This assay detects intact Osteocalcin (amino acids 1-49) as well as the N-MID fragment (amino acids 1-43).
Method
Electrochemiluminescent immunoassay (ECLIA)
Analytical Platform
Roche CobasPRO
Result Included
Osteocalcin
Specimen
Specimen Type
Serum;PlasmaContainers
Preferred Containers
Gold top (clot activator & gel);Red top (no additive)
Acceptable Containers
Lavender top (EDTA); Green top (Na Heparin); Green top (Li Heparin)
Volume
Sample Volume
1 mL
Minimum Volume
0.5 mL
Specimen Comment
Gel-separator tubes are acceptable.
Collection & Handling
Collection Instructions
Avoid hemolysis as erythrocytes contain proteases that will degrade Osteocalcin.
Handling Information
Separate within 4 hours of collection and freeze immediately. Store and send frozen
Stability
| Ambient | Refrigerated | Frozen |
|---|---|---|
| 8 hours | 3 days | 3 months |
Rejection Criteria
| Criteria | Specification |
|---|---|
| Hemolysis | Present |
Performance & Interpretation
Turnaround Time
9 days
Results
Name
Units
Reference Range
Conversion
-
Osteocalcin, N-MIDµg/LMale:
5m - 7 y: 39 - 121
8 y - 10 y: 66 - 182
11 y - 13 y: 85 - 232
14 y - 16 y: 70 - 336
17 y - 18 y: 43 - 237
19 y - 29 y: 24 - 70
30 y - 50 y: 14 - 42
≥51 y: 13 - 48
Female:
5m - 7 y: 44 - 130
8 y - 10 y: 73 - 206
11 y - 13 y: 77 - 262
14 y - 16 y: 33 - 222
17 y - 18 y: 24 - 99
≥19 y: 11 - 46
Referral Location
Canada
Interface & Setup
HL7 Interface Codes
| Order Code | Result Name | Result Codes | Units |
|---|---|---|---|
| OSTEO | N-MID Osteocalcin | 36478 | ug/L |
Test Version
Last Updated
2023-10-12