UBJ; UPE; Bence Jones protein
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Bence-Jones Protein (IFE) and Reflex Protein Electrophoresis, Urine
Test ID: BENCEJ
Test
Aliases/Synonyms
Method
Immunofixation (with reflex protein electrophoresis if Bence-Jones proteins are detected)
Report Includes
Free Kappa Light Chains; Free Lambda Light Chains; Protein; Albumin; Alpha-1 Globulin; Alpha-2 Globulin; Beta Globulin; Gamma Globulin; M-Spike
Specimens
Random Urine; 24h Urine
Clinical Utility
Urine Bence-Jones (BJ) by immunofixation is more specific for Bence-Jones proteins than protein electrophoresis (PE).
Eliminating initial PE screening enables shorter laboratory turnaround time and faster final reports.
Quantitating detected BJ proteins allows longitudinal monitoring, rather than merely indicating their presence or absence.
Test Location
Scarborough General Hospital, 3050 Lawrence Avenue, Scarborough ON M1P 2V5
Test Version
8-Aug-2023
Specimen
Specimens
Random Urine; 24h Urine
Sample Volume
15 mL
Minimum Volume
10 mL
Collection & Handling
Collection Instructions
Submit an aliquot of a random collection (first morning void preferred) or an aliquot of a 24-hour preservative-free urine collection.
Handling Information
Store and send frozen.
Additional Information
If Bence-Jones proteins are detected, protein electrophoresis will be performed, reported and billed.
Stability
| Ambient | 7 days |
|---|---|
| Refrigerated | 30 days |
| Frozen | 180 days |
Rejection Criteria
| Other | Preservatives |
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Test Version
8-Aug-2023
Performance / Interpretation
Method
Immunofixation (with reflex protein electrophoresis if Bence-Jones proteins are detected)
Turnaround Time
8 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Free Kappa Light Chains | Qualitative Test |
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| Free Lambda Light Chain | Qualitative Test |
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| Urine Protein | g/L |
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| Total Volume | L |
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| Albumin | % |
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Reported when protein electrophoresis is performed.
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| Alpha-1 Globulins | % |
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Reported when protein electrophoresis is performed.
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| Alpha-2 Globulins | % |
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Reported when protein electrophoresis is performed.
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| Beta Globulins | % |
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Reported when protein electrophoresis is performed.
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| Gamma Globulins | % |
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Reported when protein electrophoresis is performed.
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| M-Spike | % |
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Reported when protein electrophoresis is performed.
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| Interpretation | Quantitative estimation of Bence-Jones protein is reported when protein electrophoresis is performed. 24 hour Urine: Bence-Jones protein is reported as both percentage of total protein and absolute quantity (g/d and/or g/L). Random Urine: Bence-Jones protein is reported as the percentage of total protein. |
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Test Location
Scarborough General Hospital, 3050 Lawrence Avenue, Scarborough ON M1P 2V5
Test Version
8-Aug-2023
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| Multiple | 0HL7 codes |
Test Version
8-Aug-2023