Back to Test Catalogue

Estradiol – Free, Serum

Test ID: ESTDFREE S
Orderable by Ontario ND

Test

Method

LC-MS and Equilibrium Dialysis

Report Includes

Estradiol, Free; Estradiol

Specimens

Avoid gel-separator tubes; Serum

Clinical Utility

Much of Estradiol is bound to proteins. The unbound portion and Estradiol bound to proteins with low affinity reflect the free concentration. The Free Estradiol may better correlate with medical conditions than the Total Estradiol concentrations.

Test Location

Quest Diagnostics, California USA

Test Version

13-Jan-2023

Specimen

Specimens

Avoid gel-separator tubes; Serum

Collection Containers

Red top (no additive)

Sample Volume

1 mL

Minimum Volume

0.5 mL

Specimen Comment

Avoid gel-separator tubes.

Collection & Handling

Handling Information

Store and send frozen.

Stability

Ambient 24 hours
Refrigerated 7 days
Frozen 28 days

Rejection Criteria

Hemolysis Gross
Lipemia Gross
Icterus Gross
Specimen Gel separator tubes

Test Version

13-Jan-2023

Performance / Interpretation

Method

LC-MS and Equilibrium Dialysis

Turnaround Time

16 days

Results

Name Units Reference Range Conversion Factor
Estradiol, Free pg/mL
  • Adults: ≤ 0.45
Estradiol pg/mL
  • ≤ 29

Test Location

Quest Diagnostics, California USA

Test Version

13-Jan-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
ESTDFREE S 64727ESTRADIOL, FREE pg/mL
64728ESTRADIOL pg/mL

Test Version

13-Jan-2023