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Amikacin – Trough, Serum

Test ID: AMIKT

Test

Aliases/Synonyms

Aminoglycoside

Method

Kinetic Interaction of Microparticles in Solution (KIMS) Immunoassay

Platform

Roche cobas 6000

Report Includes

Amikacin - Trough

Specimens

Avoid gel separator tubes; Serum

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

2-Mar-2023

Specimen

Specimens

Avoid gel separator tubes; Serum

Collection Containers

Red top (no additive)

Sample Volume

1 mL

Minimum Volume

0.5 mL

Collection & Handling

Collection Instructions

Avoid gel-separator tubes.
For traditional dosing, collect sample 30 minutes after end of dose infusion. Wait until the third dose before collecting samples for therapeutic monitoring.
For extended dosing, after the third dose collect a sample 8 – 12 hours after end of dose infusion. Record the exact time of sampling and use nomogram for dosage adjustment if necessary.

Handling Information

Store and send frozen.

Stability

Ambient 4 hours
Refrigerated 7 days
Frozen 14 days

Rejection Criteria

Specimen Gel separator tube
Hemolysis Present
Icterus Present

Test Version

2-Mar-2023

Performance / Interpretation

Method

Kinetic Interaction of Microparticles in Solution (KIMS) Immunoassay

Platform

Roche cobas 6000

Turnaround Time

1 Day

Results

Name Units Reference Range Conversion Factor
Amikacin - Trough mg/L
  • For traditional (conventional) dosing method in adults:
     Pre-dose <8 mg/L
     Half-hour post-dose:
     20 - 30 mg/L
     For Urinary tract infection: 15 - 30 mg/L
    For extended interval (once daily) dosing method in adults:

  •  Pre-dose <1.0 mg/L
     8 - 12 hours post dose: Use nomogram for interpretation.
Refer to guidelines for Aminoglycoside dosing at your institution.

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

2-Mar-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
AMIKT 2557Amikacin, Before dose mg/L

Test Version

2-Mar-2023