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Tumour Necrosis Factor Alpha (TNF-Alpha), Plasma

Test ID: TNFA P

Test

Aliases/Synonyms

TNFa

Method

Automated fluorescence immunoassay

Platform

Proteinsimple ELLA
This analysis is performed at Hospital for Sick Children, Toronto, ON, CA

Report Includes

Tumour Necrosis Factor Alpha (TNF-Alpha);
This test is available as an individual test order or it may be ordered as part of a three-test profile Cytokine Panel 2 (TNFA, CXCL9, IFNG), Plasma. The price for the three-test profile is less than the combined total price of any two or three of these tests ordered individually.

Specimens

Plasma (EDTA)

This test is also available in serum at Quest Diagnostics - see Tumor Necrosis Factor - Alpha (TNF), Serum.

Test Location

Hospital for Sick Children, 555 University Avenue, Toronto ON M5G 1X8

Test Version

22-Jun-2022

Specimen

Specimens

Plasma (EDTA)

This test is also available in serum at Quest Diagnostics - see Tumor Necrosis Factor - Alpha (TNF), Serum.

Collection Containers

Lavender top (EDTA)

Sample Volume

0.5 mL x 2

Minimum Volume

0.25 mL x 2

Collection & Handling

Handling Information

Separate within 30 minutes of collection. Centrifuge for 15 minutes at 1000 RCF. Prepare two 0.5 mL aliquots (min 2 x 0.25 mL). Store and ship frozen.

Additional Information

Avoid freeze-thaw cycles

Stability

Ambient Unacceptable

Additional Stability Information

Avoid freeze-thaw cycles

Rejection Criteria

Hemolysis Gross
Lipemia Gross

Test Version

22-Jun-2022

Performance / Interpretation

Method

Automated fluorescence immunoassay

Platform

Proteinsimple ELLA
This analysis is performed at Hospital for Sick Children, Toronto, ON, CA

Turnaround Time

14 days

Results

Name Units Reference Range Conversion Factor
TNF-Alpha pg/mL
  • ≤9.8
Results should be interpreted in the context of the clinical presentation, therapeutic intervention, and additional laboratory data. Assay performance has not been validated in the context of treatment monitoring following administration of cytokine-blocking therapies; interpret results in this context with caution.

Test Location

Hospital for Sick Children, 555 University Avenue, Toronto ON M5G 1X8

Test Version

22-Jun-2022

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
TNFA P 64153TNF-a pg/mL
64464TNF-A Comment

Test Version

22-Jun-2022