Plasma (Na heparin)
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Oxalate, Plasma
Test ID: POXA P
Test
Method
Enzymatic-Spectrophotometric
Method Description
This is an enzymatic method based on the reduction of oxalate by oxalate oxidase. The reaction releases hydrogen peroxide, which in the presence of peroxidase reacts with a dye to give a colored end point that is measured using a BioTek EPOCH plate spectrophotometer at 590 nm. (Package insert: Trinity Biotech, Oxalate Kit, V. 07/2016)
Specimens
Test Location
Mayo Clinical Labs, Minnesota USA
Test Version
6-Jun-2023
Specimen
Specimens
Plasma (Na heparin)
Sample Volume
5 mL
Minimum Volume
2 mL
Patient Preparation
Fasting (12 hours). Patient should avoid taking vitamin C supplements for 24 hours prior to collection.
Collection & Handling
Collection Instructions
Place specimen on wet ice immediately.
Centrifuge for 10 minutes at 3,500 rpm at 4°C within 1 hour of collection and aliquot plasma into a plastic vial.
Adjust the pH of the plasma specimen to a pH of 2.3-2.7 with approximately 10 µL concentrated (12M) hydrochloric acid (or 20 µL of 6M HCl) per 1 mL plasma.
Handling Information
Store and send frozen.
Additional Information
Nonacidified specimens can be accepted if the heparinized plasma is properly frozen. However, a disclaimer will be added in nonacidified plasma: Sample was received nonacidified and frozen. In nonacidified samples oxalate values may increase spontaneously.
Stability
| Frozen | 30 days |
|---|
Test Version
6-Jun-2023
Performance / Interpretation
Method
Enzymatic-Spectrophotometric
Method Description
This is an enzymatic method based on the reduction of oxalate by oxalate oxidase. The reaction releases hydrogen peroxide, which in the presence of peroxidase reacts with a dye to give a colored end point that is measured using a BioTek EPOCH plate spectrophotometer at 590 nm. (Package insert: Trinity Biotech, Oxalate Kit, V. 07/2016)
Turnaround Time
10 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Oxalate | µmol/L |
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Reference values have not been established for patients younger than 18 years of age or older than 87 years of age.
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Test Location
Mayo Clinical Labs, Minnesota USA
Test Version
6-Jun-2023
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| POXA P | 63374Oxalate, Plasma | mcmol/L |
Test Version
6-Jun-2023