Soluble membrane attack complex (sMAC); soluble Terminal complement complex (sTCC)
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Soluble C5b-9 Complement, Plasma
Test ID: SC5B9 P
Test
Aliases/Synonyms
Method
Quantitative ELISA
Platform
MicroVue SC5b-9 Plus Enzyme Immunoassay (QUIDEL)
Report Includes
Soluble C5b-9 Complement
Specimens
Plasma
Clinical Utility
sC5b-9 is the non-cytolytic, soluble form of the terminal complement complex C5b-9 (TCC or membrane attack complex MAC). The quantitative measurement of plasma level of sC5b-9 provides information on the degree of activation of the terminal complement pathway.
sC5b-9 testing can help to confirm the diagnosis of complement-related diseases1, 2 (aHUS or C3G for example). sC5b-9 testing can also help to monitor anti-complement therapy efficiency (Eculizumab)3, 4. SC5b-9 testing should be interpreted in the context of other clinical and laboratory evidence of disease activity.
1. Bu et al. http://dx.doi.org/10.1053/j.ajkd.2015.02.326
2. Cataland et al. Blood. 2014 Jun 12;123(24):3733-8
3. Wehling et al. Clinical and Experimental Immunology, 187: 304-315
4. Jodele et al. BBMT, 2016:22,307
Test Location
QC, CA
Test Version
22-Aug-2024
Specimen
Specimens
Plasma
Collection Containers
Preferred
Plasma (EDTA lavender top)
Acceptable
Plasma (Citrate light blue top)
Sample Volume
0.6 mL x 2
Minimum Volume
0.6 mL
Collection & Handling
Handling Information
Spin to separate immediately. Submit two aliquots if possible, each over 0.5 mL. Sample processing and storage must be completed within 2 hours. Store and send frozen.
Stability
| Ambient | 4 hours |
|---|---|
| Refrigerated | 4 hours |
| Frozen | >3 months at -70°C |
Additional Stability Information
If the specimen thaws, it is unsuitable for analysis.
Rejection Criteria
| Specimen | Not frozen at -70°C |
|---|
Test Version
22-Aug-2024
Performance / Interpretation
Method
Quantitative ELISA
Platform
MicroVue SC5b-9 Plus Enzyme Immunoassay (QUIDEL)
Turnaround Time
16 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Soluble C5b-9 Complement | ng/mL |
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The sC5B-9 complex is considered a surrogate marker of terminal complement activation by all complement pathways.
Elevated concentrations of C5b-9 are associated with the development of complement-associated thrombotic microangiopathy (CA-TMA).
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Test Location
QC, CA
Test Version
22-Aug-2024
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| SC5B9 P | 63337SC5b-9 | ng/mL |
Test Version
22-Aug-2024