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Tobramycin – Trough, Serum

Test ID: TOBRAT
Test
Specimen
Performance / Interpretation
Interface / Setup

Test

Aliases/Synonyms

Aminoglycoside

Method

Competitive Binding Immunoenzymatic Assay

Platform

Roche Cobas 6000

Report Includes

Tobramycin - Trough

Specimens

Serum

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

23-Aug-2023

Specimen

Specimens

Serum

Collection Containers

Preferred

Red top (no additive)

Acceptable

Gold top (clot activator & gel)

Sample Volume

1 mL

Collection & Handling

Collection Instructions

Collect immediately before next dose. Wait until the third dose before collecting samples for therapeutic monitoring.

Handling Information

Store and send frozen.

Stability

Ambient 4 hours
Refrigerated 7 days
Frozen 30 days

Rejection Criteria

Hemolysis Grossly
Icterus Present

Test Version

23-Aug-2023

Performance / Interpretation

Method

Competitive Binding Immunoenzymatic Assay

Platform

Roche Cobas 6000

Turnaround Time

2 days

Results

Name Units Reference Range Conversion Factor
Tobramycin - Trough mg/L
  • For traditional (conventional) dosing method:
     Pre-dose: <2.0 mg/L for adults and pediatrics
     Half-hour post-dose: 4.0 - 10.0 mg/L for adults ; 5.0 - 10.0 mg/L for pediatrics
    For extended interval (once daily) dosing method:
     Pre-dose <1.0 mg/L for adults
     8 - 12 hours post-dose for adults and use nomogram for interpretation;
     3 and 6 hours post-dose for pediatrics and use nomogram for interpretation.
Refer to guidelines for Aminoglycoside dosing at your institution.

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

23-Aug-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
TOBRAT 2563Tobramycin, Before dose mg/L

Test Version

23-Aug-2023