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Tobramycin – Trough, Serum

Test ID: TOBRAT
Test
Specimen
Performance / Interpretation
Interface / Setup

Test

Aliases/Synonyms

Aminoglycoside

Method

Competitive Binding Immunoenzymatic Assay

Platform

Roche Cobas 6000

Report Includes

Tobramycin - Trough

Specimens

Serum

Test Version

23-Aug-2023

Specimen

Specimens

Serum

Collection Containers

Preferred

Red top (no additive)

Acceptable

Gold top (clot activator & gel)

Sample Volume

1 mL

Collection & Handling

Collection Instructions

Collect immediately before next dose. Wait until the third dose before collecting samples for therapeutic monitoring.

Handling Information

Store and send frozen.

Stability

Ambient 4 hours
Refrigerated 7 days
Frozen 30 days

Rejection Criteria

Hemolysis Grossly
Icterus Present

Test Version

23-Aug-2023

Performance / Interpretation

Method

Competitive Binding Immunoenzymatic Assay

Platform

Roche Cobas 6000

Turnaround Time

2 days

Results

Name Units Reference Range Conversion Factor
Tobramycin - Trough mg/L
  • For traditional (conventional) dosing method:
     Pre-dose: <2.0 mg/L for adults and pediatrics
     Half-hour post-dose: 4.0 - 10.0 mg/L for adults ; 5.0 - 10.0 mg/L for pediatrics
    For extended interval (once daily) dosing method:
     Pre-dose <1.0 mg/L for adults
     8 - 12 hours post-dose for adults and use nomogram for interpretation;
     3 and 6 hours post-dose for pediatrics and use nomogram for interpretation.
Refer to guidelines for Aminoglycoside dosing at your institution.

Test Version

23-Aug-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
TOBRAT 2563Tobramycin, Before dose mg/L

Test Version

23-Aug-2023