Methotrexate
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Methotrexate, Serum/Plasma
Test ID: METHO
Test
Method
Competitive Binding Immunoenzymatic Assay
Report Includes
Specimens
Gel-separator tubes are not acceptable; Serum; Plasma
Clinical Utility
Refer to BC Cancer Methotrexate Monograph and Cancer Care Ontario at external websites.
Test Location
St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8
Test Version
18-Sep-2023
Specimen
Specimens
Gel-separator tubes are not acceptable; Serum; Plasma
Collection Containers
Preferred
Red Top (no additive)
Acceptable
Green top (Na Heparin); Green top (Li Heparin); Lavender top (EDTA)
Sample Volume
1 mL
Minimum Volume
0.5 mL
Specimen Comment
Avoid gel separator tubes.
Collection & Handling
Collection Instructions
Collect at 24, 48 and 72 hrs following high I.V. dose.
Handling Information
Separate within 2 hours of collection. Specimen must be labelled inside and outside of the light protecting wrap. Store and submit specimen protected from light in an amber vial.
Additional Information
The sampling of Methotrexate is dependent on dose, duration of infusion and clinical status of the patient.
Stability
| Ambient | 2 days |
|---|---|
| Refrigerated | 14 days |
| Frozen | 1 month |
Rejection Criteria
| Specimen | Not protected from light or collected with gel-separator |
|---|
Test Version
18-Sep-2023
Performance / Interpretation
Method
Competitive Binding Immunoenzymatic Assay
Turnaround Time
2 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Methotrexate | µmol/L |
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Refer to BC Cancer Methotrexate Monograph and Cancer Care Ontario at external websites.
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Test Location
St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8
Test Version
18-Sep-2023
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| METHO | 3163Methotrexate | umol/L |
Test Version
18-Sep-2023