LA; Non-Specific Inhibitor; NSI; Non Specific Inhibitor; Circulating Anticoagulant; Phospholipid Antibody; Antiphospholipid; APL; Diluted Russell Viper Venom Time
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Lupus Anticoagulant, Plasma
Test ID: NSCI
Test
Aliases/Synonyms
Method
Lupus Anticoagulant-Sensitive APTT Reagent/RVV Time Test
Method Description
LA testing is done by optical clot detection methods, using the HemosIL Silica Clotting Time and dRVVT Screen and Confirm reagents on the ACL TOP analyzer.
Report Includes
INR; Prothorombin Time; Thrombin time; Fibrinogen; APTT; Lupus Anticoagulant Interpretation; SCT; DRVVT
Specimens
Plasma
Clinical Utility
Testing is done in accordance with recent International Society on Thrombosis and Haemostasis (ISTH) guidelines on lupus anticoagulant (LA), that recommend:
- assessment of the PT, APTT, thrombin clotting time (TCT) and fibrinogen as part of LA test panel to evaluate for potential interference from anticoagulants or a coagulopathy
- testing for LA by a LA-sensitive APTT and a dilute Russel Viper Venom Time (dRVVT)
- mixing and confirmatory tests (for phospholipipd dependence) if the LA screening tests are abnormal.
Test Location
Laboratory Reference Centre, 237 Barton Street E, Hamilton ON L8L 2X2
Test Version
15-May-2024
Specimen
Specimens
Plasma
Collection Containers
Blue top (3.2% buffered citrate)
Sample Volume
1 mL
Collection & Handling
Collection Instructions
Ensure full draw with 9:1 ratio (9 parts blood to 1 part anticoagulant).
Handling Information
Prepare platelet-poor plasma (double spun) within 4 hours of collection and freeze immediately. Store and send frozen.
Stability
| Ambient | Unacceptable |
|---|---|
| Refrigerated | Unacceptable |
| Frozen | 60 days |
Additional Stability Information
Stable up to twelve months at -70°C
Rejection Criteria
| Specimen | Not frozen |
|---|
Test Version
15-May-2024
Performance / Interpretation
Method
Lupus Anticoagulant-Sensitive APTT Reagent/RVV Time Test
Method Description
LA testing is done by optical clot detection methods, using the HemosIL Silica Clotting Time and dRVVT Screen and Confirm reagents on the ACL TOP analyzer.
Turnaround Time
9 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| INR |
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| Prothrombin Time (PT) in PPP by Coagulation Assay | s |
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| Thrombin Time | s |
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| Fibrinogen (g/L) in PPP by Coagulation Assay | g/L |
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| APPT | s |
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| Lupus Anticoagulant Interpretation |
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| SCT Screen |
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| SCT Confirm |
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Only reported when screen is abnormal
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| SCT SC Ratio |
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Only reported when screen is abnormal
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| SCT MIX |
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Only reported when screen is abnormal
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| DRVVT screen |
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| DRVVT Confirm |
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Only reported when screen is abnormal
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| DRVVT SC Ratio |
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Only reported when screen is abnormal
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| DRVVT Mix |
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Only reported when screen is abnormal
|
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Test Location
Laboratory Reference Centre, 237 Barton Street E, Hamilton ON L8L 2X2
Test Version
15-May-2024
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| NSCI | 64493INR | |
| 64494PROTHROMBIN TIME (PT) IN PPP BY COAGULATION ASSAY | s | |
| 64481THROMBIN TIME | s | |
| 7395FIBRINOGEN (G/L) IN PPP BY COAGULATION ASSAY | g/L | |
| 64504APTT | s | |
| 64503LUPUS ANTICOAGULANT INTERPRETATION | ||
| 64495SCT SCREEN | ||
| 64496SCT CONFIRM | ||
| 64497SCT SC RATIO | ||
| 64498SCT MIX | ||
| 64499DRVVT SCREEN | ||
| 64500DRVVT CONFIRM | ||
| 64501DRVVT SC RATIO | ||
| 64502DRVVT MIX |
Test Version
15-May-2024