Hb Fractionation; Hemoglobin Electrophoresis; Hemoglobinopathy Investigation
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Hemoglobin Fractionation, Blood
Test ID: HBELEC
Test
Aliases/Synonyms
Method
Capillary Electrophoresis
Method Description
Includes hemoglobin electrophoresis gel card and HbH preparation if necessary based on diagnostic criteria.
Platform
Sebia Capillarys 2 Flex
Report Includes
Hemoglobin A2; Hemoglobin F; Hemoglobin A; Sickle Cell Screen; Hemoglobin Variants; Interpretation
Specimens
Whole Blood
Test Location
University Health Network, 200 Elizabeth St, 11E-444, Toronto ON M5G 2C4
Test Version
18-Aug-2023
Specimen
Specimens
Whole Blood
Collection Containers
Lavender top (EDTA); Yellow top (ACD)
Sample Volume
4 mL
Minimum Volume
3 mL
Collection & Handling
Handling Information
Frozen specimens are unacceptable – do not freeze. Store and send cold.
Additional Information
You must provide Hemoglobin, MCV, RDW, date of birth and racial origin of the patient. This information may not be included in the report but is required for interpretation of findings (included in report). Hemoglobin H (HbH) screen may not be done unless MCV is less than 75. Hemolyzed specimens may yield uninterpretable results.
Stability
| Refrigerated | 7 days |
|---|---|
| Frozen | Unacceptable |
Rejection Criteria
| Specimen | Frozen |
|---|---|
| Other | Missing information (Hemoglobin, MCV, RDW, date of birth and racial origin of the patient) |
Test Version
18-Aug-2023
Performance / Interpretation
Method
Capillary Electrophoresis
Method Description
Includes hemoglobin electrophoresis gel card and HbH preparation if necessary based on diagnostic criteria.
Platform
Sebia Capillarys 2 Flex
Turnaround Time
9 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Hemoglobin A | % |
|
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| Hemoglobin F | % |
|
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| Hemoglobin A2 | % |
|
||
Test Location
University Health Network, 200 Elizabeth St, 11E-444, Toronto ON M5G 2C4
Test Version
18-Aug-2023
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| HBELEC | 8698Hb A | % |
| 8697Hb F | % | |
| 8696Hb A2 | % | |
| 8694Sickle Cell Screen | ||
| 8695Hb H Preparation | ||
| 63543Cellulose Acetate | ||
| 40002Tech Comment | ||
| 65163Hemoglobin (G/L) in blood | g/L | |
| 65164MCV (FL) | fL | |
| 65165RDW-CV | % | |
| 65166HBS | % | |
| 65167HBC | % | |
| 65168HBE | % | |
| 65169HB LEPORE | % | |
| 65170HBD | % | |
| 65171HBH | % | |
| 65172HBG | % | |
| 65173HBJ | % | |
| 65174 Unidentified Hb | % | |
| 38470Hematologist Interpretation |
Test Version
18-Aug-2023