Test Overview
Clinical Utility
17-Hydroxypregnenolone is useful in the diagnosis of 3-Beta-Hydroxylase enzyme deficiency, a rare cause of congenital adrenal hyperplasia, and 17-Hydroxylase (P450c17) enzyme deficiency.
Method
Chromatography/Mass Spectrometry
Result Included
17 Hydroxypregnenolone
Aliases/Synonyms
17 Hydroxypregnenolone; 17OH-Pregnenolone
Specimen
Specimen Type
SerumContainers
Collection Containers
Red top (no additive)
Volume
Sample Volume
2.0 mL
Minimum Volume
0.4 mL
Collection & Handling
Handling Information
Store and send frozen.
Stability
| Ambient | Refrigerated | Frozen |
|---|---|---|
| 24 hours | 5 days | 28 days |
Rejection Criteria
| Criteria | Specification |
|---|---|
| Hemolysis | Gross |
| Lipemic | Gross |
| Icteric | Gross |
| Specimen | Gel separator tube |
Performance & Interpretation
Turnaround Time
11 days
Results
Name
Units
Reference Range
Conversion
-
17 Hydroxypregnenoloneng/dLAdult Males: ≤ 700
Adult Females, Pre-Menopausal:
Mid Follicular: 38 - 378
Surge: 33 - 583
Mid Luteal: 70 - 470
Pediatric Male and Female::
< 30 days: ≤ 3013
1-11 months: ≤ 869
1 year: ≤ 557
2 years: ≤ 487
3 years: ≤ 541
4 years: ≤ 561
5 years: ≤ 564
6 years: ≤ 561
7 years: ≤ 549
8 years: ≤ 569
9 years: ≤ 590
10 years: ≤ 594
11 years: ≤ 629
12 years: ≤ 666
13 years: ≤ 678
14 years: ≤ 706
15 years: ≤ 735
16 years: ≤ 739
17 years: ≤ 756
Referral Location
Out-of-Country
Interface & Setup
HL7 Interface Codes
| Order Code | Result Name | Result Codes | Units |
|---|---|---|---|
| 17OHPL | 17 HYDROXYPREGNENOLONE | 62051 | ng/dL |
Test Version
Last Updated
2025-11-19