Glucagon
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Glucagon, Plasma
Test ID: GLP
Test
Method
Extraction/Immunoassay
Method Description
Big plasma glucagon (BPG), which is considered to be biologically inactive, is extracted using ethanol prior to assay of the specimen. This enables accurate measurement of the biologically active glucagon in the specimen.(Hendriks T: Radioimmunoassay of clinically meaningful components of circulating glucagon: removal of big plasma glucagon prior to assay. Clin Chim Acta 1984;140:301-307) Following ethanol extraction, glucagon reacts with an antiglucagon antibody that is attached to tiny polystyrene beads. After incubation and washing, a second detection antibody is added and attaches to any bead-bound glucagon, forming a sandwich assay. A streptavidin-phycoerythrin (SPE) tag binds to the glucagon-antibody complex. Laser-based fluorescent analysis of the resulting glucagon-antibody-SPE complex is performed on the Luminex 200 instrument.(Package insert: Human Endocrine LINCOplex Kit, HENDO-65K-Rev. 03/03/06)
Platform
Luminex 200
Report Includes
Specimens
Plasma (EDTA)
Test Location
Mayo Clinical Labs, Minnesota USA
Test Version
30-Sep-2019
Specimen
Specimens
Plasma (EDTA)
Sample Volume
2 mL
Minimum Volume
0.45 mL
Patient Preparation
Fasting.
Collection & Handling
Collection Instructions
Collect fasting specimen in pre-chilled tube and then chill tube in wet ice for 10 minutes.
Handling Information
Centrifuge in a refrigerated centrifuge or in chilled centrifuge cup. Immediately after centrifugation, separate plasma in a plastic transport vial and freeze. Store and send frozen.
Stability
| Frozen | 90 days |
|---|
Test Version
30-Sep-2019
Performance / Interpretation
Method
Extraction/Immunoassay
Method Description
Big plasma glucagon (BPG), which is considered to be biologically inactive, is extracted using ethanol prior to assay of the specimen. This enables accurate measurement of the biologically active glucagon in the specimen.(Hendriks T: Radioimmunoassay of clinically meaningful components of circulating glucagon: removal of big plasma glucagon prior to assay. Clin Chim Acta 1984;140:301-307) Following ethanol extraction, glucagon reacts with an antiglucagon antibody that is attached to tiny polystyrene beads. After incubation and washing, a second detection antibody is added and attaches to any bead-bound glucagon, forming a sandwich assay. A streptavidin-phycoerythrin (SPE) tag binds to the glucagon-antibody complex. Laser-based fluorescent analysis of the resulting glucagon-antibody-SPE complex is performed on the Luminex 200 instrument.(Package insert: Human Endocrine LINCOplex Kit, HENDO-65K-Rev. 03/03/06)
Platform
Luminex 200
Turnaround Time
7 days
Specimen Retention Time
90 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Glucagon | pg/mL |
|
||
Test Location
Mayo Clinical Labs, Minnesota USA
Test Version
30-Sep-2019
Interface / Setup
Test Version
30-Sep-2019