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Glucagon, Plasma

Test ID: GLP

Test

Method

Extraction/Immunoassay

Method Description

Big plasma glucagon (BPG), which is considered to be biologically inactive, is extracted using ethanol prior to assay of the specimen. This enables accurate measurement of the biologically active glucagon in the specimen.(Hendriks T: Radioimmunoassay of clinically meaningful components of circulating glucagon: removal of big plasma glucagon prior to assay. Clin Chim Acta 1984;140:301-307) Following ethanol extraction, glucagon reacts with an antiglucagon antibody that is attached to tiny polystyrene beads. After incubation and washing, a second detection antibody is added and attaches to any bead-bound glucagon, forming a sandwich assay. A streptavidin-phycoerythrin (SPE) tag binds to the glucagon-antibody complex. Laser-based fluorescent analysis of the resulting glucagon-antibody-SPE complex is performed on the Luminex 200 instrument.(Package insert: Human Endocrine LINCOplex Kit, HENDO-65K-Rev. 03/03/06)

Platform

Luminex 200

Report Includes

Glucagon

Specimens

Plasma (EDTA)

Test Location

Mayo Clinical Labs, Minnesota USA

Test Version

30-Sep-2019

Specimen

Specimens

Plasma (EDTA)

Sample Volume

2 mL

Minimum Volume

0.45 mL

Patient Preparation

Fasting.

Collection & Handling

Collection Instructions

Collect fasting specimen in pre-chilled tube and then chill tube in wet ice for 10 minutes.

Handling Information

Centrifuge in a refrigerated centrifuge or in chilled centrifuge cup. Immediately after centrifugation, separate plasma in a plastic transport vial and freeze. Store and send frozen.

Stability

Frozen 90 days

Test Version

30-Sep-2019

Performance / Interpretation

Method

Extraction/Immunoassay

Method Description

Big plasma glucagon (BPG), which is considered to be biologically inactive, is extracted using ethanol prior to assay of the specimen. This enables accurate measurement of the biologically active glucagon in the specimen.(Hendriks T: Radioimmunoassay of clinically meaningful components of circulating glucagon: removal of big plasma glucagon prior to assay. Clin Chim Acta 1984;140:301-307) Following ethanol extraction, glucagon reacts with an antiglucagon antibody that is attached to tiny polystyrene beads. After incubation and washing, a second detection antibody is added and attaches to any bead-bound glucagon, forming a sandwich assay. A streptavidin-phycoerythrin (SPE) tag binds to the glucagon-antibody complex. Laser-based fluorescent analysis of the resulting glucagon-antibody-SPE complex is performed on the Luminex 200 instrument.(Package insert: Human Endocrine LINCOplex Kit, HENDO-65K-Rev. 03/03/06)

Platform

Luminex 200

Turnaround Time

7 days

Specimen Retention Time

90 days

Results

Name Units Reference Range Conversion Factor
Glucagon pg/mL
  • ≤6 h: 100 - 650
  • 1 - 2 d: 70-450
  • 2 - 4 d: 100-650
  • 4 - 14 d: Declining gradually to adult range
  • >14 d: ≤80 (Based on 95% confidence limits)

Test Location

Mayo Clinical Labs, Minnesota USA

Test Version

30-Sep-2019

Interface / Setup

Test Version

30-Sep-2019