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Cucumber (f244) IgE, Serum

Test ID: CUCUMBER
Orderable by Ontario ND

Test

Method

ImmunoCAP™

Method Description

ImmunoCAP™ assays are based on binding of allergen-specific IgE antibodies in serum to allergen (extract or allergen molecule) coupled in excess to a solid phase. Bound IgE is subsequently detected with enzyme-labeled anti-human IgE, where the fluorescence intensity is proportional to the amount of allergen-specific IgE.

Platform

Phadia

Report Includes

Cucumber (f244) IgE

Specimens

Serum

Clinical Utility

In patients suffering from extrinsic asthma, hay fever or atopic eczema, symptoms develop after exposure to specific allergens. This immediate (atopic or anaphylactic) type of allergy is a function of a special type of serum antibodies belonging to the IgE class of immunoglobulins.

Clinician must determine clinical significance of specific IgE result after correlation with clinical exam and history. Detectable levels are present in patients with allergic disease and in approx. 15% of symptomatic healthy persons. Some with classic atopic symptoms may not have detectable levels. Specific IgE may decrease with time and lack of exposure to allergens.

Test Location

Hamilton General Hospital, 237 Barton St E, Hamilton ON L8L 2X2

Test Version

17-Nov-2023

Specimen

Specimens

Serum

Sample Volume

1 mL

Specimen Comment

Submit enough specimen for number of IgE tests ordered. Each test requires 50 µL and 2 mL is sufficient for 10-12 tests.

Collection & Handling

Handling Information

Store and send frozen.

Additional Information

Stability

Refrigerated 3 days
Frozen 6 Months

Test Version

17-Nov-2023

Performance / Interpretation

Method

ImmunoCAP™

Method Description

ImmunoCAP™ assays are based on binding of allergen-specific IgE antibodies in serum to allergen (extract or allergen molecule) coupled in excess to a solid phase. Bound IgE is subsequently detected with enzyme-labeled anti-human IgE, where the fluorescence intensity is proportional to the amount of allergen-specific IgE.

Platform

Phadia

Turnaround Time

10 days

Results

Name Units Reference Range Conversion Factor
Cucumber (f244), IgE KU/L
  • <0.35

Clinician must determine clinical significance of specific IgE result after correlation with clinical exam and history. Detectable levels are present in patients with allergic disease and in approx. 15% of symptomatic healthy persons. Some with classic atopic symptoms may not have detectable levels. Specific IgE may decrease with time and lack of exposure to allergens.
Use caution when interpreting drug, venom and potential anaphylactic allergen results.

Specific IgEClassInterpretive Comments
< 0.35kU/L0Absent or undetectable allergen specific IgE
0.35 - 0.69kU/L1Low levels of allergen specific IgE
0.70 - 3.49kU/L2Moderate levels of allergen specific IgE
3.50 - 17.49kU/L3High Levels of allergen specific IgE
17.50 - 49.99kU/L4Very High levels of allergen specific IgE
50.00 - 100.00kU/L5Very High levels of allergen specific IgE
> 100.00kU/L6Extremely High levels of allergen specific IgE

Test Location

Hamilton General Hospital, 237 Barton St E, Hamilton ON L8L 2X2

Test Version

17-Nov-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
CUCUMBER 31034IgE Cucumber kU/L
31999IgE Allergen Interpretation

Test Version

17-Nov-2023