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5-Hydroxyindole Acetic Acid (5-HIAA), Random Urine

Test ID: 5HIAARU
Orderable by Ontario ND

Test

Aliases/Synonyms

5HIAA

Method

LC-MS/MS

Report Includes

Creatinine; 5-Hydroxyindole Acetic Acid; 5-Hydroxyindole Acetic Acid/Creatinine Ratio

Specimens

Urine (Random)

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

16-Nov-2022

Specimen

Specimens

Urine (Random)

Collection Containers

Mid-stream urine container

Sample Volume

10 mL

Patient Preparation

The following foods and medications should be avoided for three (3) days prior to the collection of urine, and during the collection of the sample:
Foods containing high hydroxyindole concentrations (e.g. aubergines, avocados, bananas, black- and red-currants, kiwi, gooseberries, melons, tomatoes, plums, pineapples and walnuts) as well as Glyceryl Guaiacolate (an ingredient in some cough medicines) can increase urinary 5-HIAA excretion. Pregnancy and sleep deprivation can also result in raised 5-HIAA excretion.
Medications and drugs such as Levodopa, Methyldopa, Monoamine Oxidase Inhibitors, Ethanol, Imipramine, Isoniazid, Reserpine and p-Chlorophenylalanine can reduce 5- HIAA production. Others, such as Acetanilide, Phenacetin, Methocarbamol, Cisplatin, Fluorouracil, Melphalan and Rauwolfia Alkaloids can increase urinary 5-HIAA, as can 5- Hydroxytryptophan, which is used to treat depression and is available over-the-counter.

Collection & Handling

Handling Information

Acidify sample within 24 hours of collection using 6N HCl to a pH of less than 4 (just one or 2 drops).

Additional Information

Indicate Random.

Stability

Refrigerated 1month
Frozen > 1 month

Rejection Criteria

Specimen pH > 5.0

Test Version

16-Nov-2022

Performance / Interpretation

Method

LC-MS/MS

Turnaround Time

10 days

Results

Name Units Reference Range Conversion Factor
Creatinine mmol/L
  • Not available for random urine
5-Hydroxyindole Acetic Acid µmol/L
  • Not available for random urine
5-Hydroxyindole Acetic Acid/Creatinine Ratio µmol/mmol Creatinine
  • Less than 5.0

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

16-Nov-2022

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
5HIAARU 28004Creatinine Rand Urine mmol/L
280105-HIAA Random umol/L
280135HIAA Creat Ratio umol/mmol crea

Test Version

16-Nov-2022