Total Complement Function Assay
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Complement – Total (CH50), Serum
Test ID: CH50
Test
Aliases/Synonyms
Method
Spectrophotometry
Report Includes
Complement - Total (CH50)
Specimens
Serum
Clinical Utility
CH50 is a screen for complement deficiencies or significant activation. Measures the relative activity of the total classical pathway (C1-C9).
Complement activity is very labile and reliable results depend on correct handling of the specimen.
Test Location
London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5
Test Version
22-Apr-2020
Specimen
Specimens
Serum
Sample Volume
2 mL
Minimum Volume
0.5 mL
Collection & Handling
Handling Information
Allow filled tube to clot at 22 – 37°C for 30 – 60 minutes. Centrifuge at room temperature. Aliquot into storage tube and freeze as soon as possible. Store and send frozen.
Additional Information
CH50 may be decreased by: delayed separation of serum, insufficient clotting, prolonged storage at room temperature, use of plasma, presence of immune complexes, cryoglobulins, bacteria or particulates.
Stability
| Frozen | 14 days |
|---|
Additional Stability Information
Stable at least 30 days when stored at -80°C
Rejection Criteria
| Hemolysis | Gross |
|---|---|
| Lipemia | Present |
| Specimen | Plasma |
Test Version
22-Apr-2020
Performance / Interpretation
Method
Spectrophotometry
Turnaround Time
5 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Complement - Total | U/mL |
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Analytical range 14 - 105 U/mL. Results less than or greater than these limits will be reported as such.
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Test Location
London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5
Test Version
22-Apr-2020
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| CH50 | 8787CH50 | U/mL |
Test Version
22-Apr-2020