Test Overview
Method
Electrochemiluminescent immunoassay (ECLIA)
Analytical Platform
INOVA Bioflash
Method Description
One-step, semi-quantitative chemiluminescent immunoassay
Result Included
β-2-Glycoprotein 1 Antibodies IgG; β-2-Glycoprotein 1 Antibodies IgM
Aliases/Synonyms
Phospholipid Antibody; Antiphospholipid; APL; Beta 2 GP1 Ab IgG; Beta 2 GP1 Ab IgG; Beta 2 Glycoprotein 1 Antibodies
Specimen
Specimen Type
SerumContainers
Collection Containers
Gold top (clot activator & gel)
Volume
Sample Volume
1 mL
Minimum Volume
0.5 mL
Specimen Comment
Plasma is not acceptable
Collection & Handling
Handling Information
Separate and freeze immediately. Store and send frozen.
Stability
| Ambient | Refrigerated | Frozen |
|---|---|---|
| 24 hours | 48 hours | > 48 hours |
If the specimen thaws, it is unsuitable for analysis.
Rejection Criteria
| Criteria | Specification |
|---|---|
| Specimen | Not frozen or plasma |
Performance & Interpretation
Turnaround Time
20 days
Results
-
β-2-Glycoprotein 1 Antibodies IgGCU≤ 20
-
β-2-Glycoprotein 1 Antibodies IgMCU≤ 20
Comment
Results ≤ 20 CU are considered Negative
Results > 20 CU are considered Positive
The 20 CU positive cut-off corresponds to the upper 99th percentile as recommended by the International Committee in Sydney (International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost 2006, 4:295-306)
Referral Location
Canada
Interface & Setup
HL7 Interface Codes
| Order Code | Result Name | Result Codes | Units |
|---|---|---|---|
| B2GLYAB | B2GP1 IgG | 62957 | CU |
| B2GP1 IgM | 62958 | CU |
Test Version
Last Updated
2025-03-26