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Dihydrotestosterone (DHT) LC-MS/MS, Serum/Plasma

Test ID: DHTSM SP

Test

Aliases/Synonyms

Dihydro-Testosterone

Method

LC-MS/MS

Method Description

DHT is measured by liquid chromatography mass spectrometry (LC-MS/MS) for the highest level of analytical sensitivity and specificity. The common interferences that affect immunoassays (i.e., heterophile antibodies, human anti-mouse antibodies, or cross-reactivity to other similar structured steroids) are absent in this LC-MS/MS method.

Report Includes

Dihydrotestosterone

Specimens

Serum; Plasma

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

5-Apr-2023

Specimen

Specimens

Serum; Plasma

Collection Containers

Preferred

Gold top (clot activator & gel)

Acceptable

Lavender top (EDTA); Green top (sodium heparin); Green top (lithium heparin)

Sample Volume

1 mL

Minimum Volume

450 µL

Collection & Handling

Handling Information

Store and send frozen.

Stability

Refrigerated 7 days
Frozen 3 months

Additional Stability Information

Stable up to 6 freeze/thaws.

Rejection Criteria

Hemolysis Gross

Test Version

5-Apr-2023

Performance / Interpretation

Method

LC-MS/MS

Method Description

DHT is measured by liquid chromatography mass spectrometry (LC-MS/MS) for the highest level of analytical sensitivity and specificity. The common interferences that affect immunoassays (i.e., heterophile antibodies, human anti-mouse antibodies, or cross-reactivity to other similar structured steroids) are absent in this LC-MS/MS method.

Turnaround Time

8 days

Results

Name Units Reference Range Conversion Factor
Dihydrotestosterone nmol/L
  • Male:
  • Cord Blood: ≤ 0.34
  • ≤ 6 months: ≤ 4.13
  • Tanner Stages:
  • Stage I (> 6 months and prepubertal): ≤ 0.17
  • Stage ii: ≤ 0.69
  • Stage III: 0.28 - 1.14
  • Stage IV: 0.76 - 1.79
  • Stage V: 0.83 - 2.24
  • > 19 years: 0.39 - 3.29
  • Female:
  • Cord Blood: ≤ 0.17
  • ≤ 6 months: ≤ 4.13
  • Tanner Stages:
  • Stage I (> 6 months and prepubertal): ≤ 0.17
  • Stage II - V: ≤ 1.03
  • 20 - 55 years ≤ 1.03
  • > 55 years ≤ 0.44

Test Location

St. Michael's Hospital, 30 Bond Street, Toronto ON M5B 1W8

Test Version

5-Apr-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
DHTSM SP 64528Dihydrotestosterone nmol/L

Test Version

5-Apr-2023