Test ID: DHTSM SP

Dihydrotestosterone (DHT) LC-MS/MS, Serum/Plasma

Test Overview

Method

Liquid chromatography-tandem mass spectrometry (LC-MS/MS)

Method Description

DHT is measured by liquid chromatography mass spectrometry (LC-MS/MS) for the highest level of analytical sensitivity and specificity. The common interferences that affect immunoassays (i.e., heterophile antibodies, human anti-mouse antibodies, or cross-reactivity to other similar structured steroids) are absent in this LC-MS/MS method.

Result Included

Dihydrotestosterone

Aliases/Synonyms

Dihydro-Testosterone

Specimen

Specimen Type

Serum
Plasma

Containers

Preferred Containers

Gold top (clot activator & gel)

Acceptable Containers

Lavender top (EDTA); Green top (sodium heparin); Green top (lithium heparin)

Volume

Sample Volume

1 mL

Minimum Volume

450 µL

Collection & Handling

Handling Information

Store and send frozen.

Stability

Refrigerated	Frozen
7 days	3 months

Stable up to 6 freeze/thaws.

Rejection Criteria

Criteria	Specification
Hemolysis	Gross

Performance & Interpretation

Turnaround Time

8 days

Results

Dihydrotestosterone

nmol/L

Male:
Cord Blood: ≤ 0.34
≤ 6 months: ≤ 4.13
Tanner Stages:
Stage I (> 6 months and prepubertal): ≤ 0.17
Stage ii: ≤ 0.69
Stage III: 0.28 - 1.14
Stage IV: 0.76 - 1.79
Stage V: 0.83 - 2.24
> 19 years: 0.39 - 3.29
Female:
Cord Blood: ≤ 0.17
≤ 6 months: ≤ 4.13
Tanner Stages:
Stage I (> 6 months and prepubertal): ≤ 0.17
Stage II - V: ≤ 1.03
20 - 55 years ≤ 1.03
> 55 years ≤ 0.44

Referral Location

Canada

Interface & Setup

HL7 Interface Codes

Order Code	Result Name	Result Codes	Units
DHTSM SP	Dihydrotestosterone	64528	nmol/L

Test Version

Last Updated

2023-04-05