Phosphoserine; Hydroxyproline; Histidine; Phosphoethanolamine; Asparagine; 1-Methylhistidine; Taurine; 3-Methylhistidine; Serine; Glutamine; Carnosine; Arginine; Glycine; Anserine; EA/ARS/SAC (Ethanolamine/Arginosuccinic acid/Saccharopine); Aspartate; Sarcosine; Glutamate; Citrulline; B-alanine; Threonine; Alanine; GABA/Homocitrulline; Aminoadipic Acid; Proline; B-aminobutyric Acid; hydroxylysine 1; Hydroxylysine 2; a-aminobutyric acid; Cystathionine; Ornithine; Cystine; Lysine; Tyrosine; Methionine; Valine; Isoleucine; Alloisoleucine; Leucine; Phenylalanine; Tryptophan
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Amino Acids Quantitation, Serum/Plasma
Test ID: AAQ
Test
Method
UPLC absorbance
Report Includes
Specimens
Serum;Plasma
Clinical Utility
Quantitative analysis of conventional proteinogenic and nonstandard amino acids is important for the detection and monitoring of patients with inborn errors of metabolism. Additionally, knowledge of amino acid concentrations provides useful information when determining nutritional status following total parenteral nutrition, surgery and shock trauma treatment.
For cerebral aminoacidopathies and nonketotic hyperglycemia (NKH), plasma/serum sample should accompany the CSF sample and both tests must be requested on requisition.
For cerebral aminoacidopathies and nonketotic hyperglycemia (NKH), plasma/serum sample should accompany the CSF sample and both tests must be requested on requisition.
Test Location
Hospital for Sick Children, 555 University Avenue, Toronto ON M5G 1X8
Test Version
18-Sep-2023
Specimen
Specimens
Serum;Plasma
Collection Containers
Preferred
Green Top (Li Heparin); Green top (Na Heparin)
Acceptable
Red Top (no additive)
Sample Volume
1 mL
Minimum Volume
200 uL
Collection & Handling
Collection Instructions
If submitting serum/plasma and CSF for analysis, specimens should be collected within an hour of each other to facilitate interpretation of findings.
Handling Information
Separate and freeze within 1 hour. Store and send frozen.
Stability
| Frozen | 30 days |
|---|
Rejection Criteria
| Specimen | Capillary |
|---|
Test Version
18-Sep-2023
Performance / Interpretation
Method
UPLC absorbance
Turnaround Time
8 days
Results
| Name | Units | Reference Range | Conversion Factor |
|---|---|---|---|
| Amino Acids (Various) | µmol/L |
|
Test Location
Hospital for Sick Children, 555 University Avenue, Toronto ON M5G 1X8
Test Version
18-Sep-2023
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| AAQ | 62350Phosphoserine | umoll/L |
| 62351Hydroxyproline | umol/L | |
| 22843Histidine | umol/L | |
| 62352Phosphoethanolamine | umol/L | |
| 62353Asparagine | umol/L | |
| 623541-Methylhistidine | umol/L | |
| 62355Taurine | umol/L | |
| 623563-Methylhistidine | umol/L | |
| 22851Serine | umol/L | |
| 22855Glutamine | umol/L | |
| 62357Carnosine | umol/L | |
| 22837Arginine | umol/L | |
| 22842Glycine | umol/L | |
| 62358Anserine | umol/L | |
| 62359Ethanolamine/ARS/Saccharopine | umol/L | |
| 22838Aspartate | umol/L | |
| 62360Sarcosine | umol/L | |
| 22841Glutamate | umol/L | |
| 22839Citrulline | umol/L | |
| 62361Beta-Alanine | umol/L | |
| 22852Threonine | umol/L | |
| 22836Alanine | umol/L | |
| 62362GABA/Homocitrulline | umol/L | |
| 62363Alpha-aminoadipic acid | umol/L | |
| 22850Proline | umol/L | |
| 62364Beta-aminoisobutyric acid | umol/L | |
| 62365Hydroxylysine 1 | umol/L | |
| 62366Hydroxylysine 2 | umol/L | |
| 62367Alpha-amino-n-butyric acid | umol/L | |
| 62368Cystathionine | umol/L | |
| 22848Ornithine | umol/L | |
| 22840Cystine | umol/L | |
| 22846Lysine | umol/L | |
| 22853Tyrosine | umol/L | |
| 22847Methionine | umol/L | |
| 22854Valine | umol/L | |
| 22844Isoleucine | umol/L | |
| 39540Alloisoleucine | umol/L | |
| 22845Leucine | umol/L | |
| 22849Phenylalanine | umol/L | |
| 62369Tryptophan | umol/L | |
| 39544Amino Acid Comment |
Test Version
18-Sep-2023