Nicotinamide; Nicotinic Acid; Niacin
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Vitamin B3, Serum/Plasma
Test ID: VITB3
Test
Aliases/Synonyms
Method
Chromatography/Mass Spectrometry
Report Includes
Nicotinamide; Nicotinic Acid
Specimens
Gel-separator tubes are not acceptable; Serum; Plasma
Test Location
Quest Diagnostics, California USA
Test Version
20-Sep-2022
Specimen
Specimens
Gel-separator tubes are not acceptable; Serum; Plasma
Collection Containers
Preferred
Red top (No additive)
Acceptable
Lavender top (EDTA)
Sample Volume
1.0 mL
Minimum Volume
0.5 mL
Specimen Comment
Avoid gel-separator tubes.
Collection & Handling
Handling Information
Store and submit specimen protected from light in an amber vial.
Store and send frozen.
Stability
| Ambient | Unacceptable |
|---|---|
| Refrigerated | Unacceptable |
| Frozen | 30 days |
Rejection Criteria
| Specimen | Gel tube or not protected from light |
|---|
Test Version
20-Sep-2022
Performance / Interpretation
Method
Chromatography/Mass Spectrometry
Turnaround Time
7 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Nicotinamide | ng/mL |
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Nicotinamide is a metabolite of nicotinic acid. Due to the large variability in the metabolism of nicotinic acid, plasma concentrations of this metabolite are variable. In one study, fasting plasma concentrations were reported to be approximately 40 ng/mL. In another study it was reported that the administration of a single 1000 mg of extended-release tablet of nicotinic acid resulted in a mean peak nicotinamide concentration of 400 ng/mL between 5 and 10 hours post dose, decreasing to about 100 ng/mL by 16 hours post dose.
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| Nicotinic Acid | ng/mL |
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Due to the large variability in the metabolism of nicotinic acid, the dosing preparation used (immediate-release vs. extended release), and the mg doses used, the serum concentrations may range from less than 20 ng/mL to about 30,000 ng/mL. After oral administration of an immediate-release tablet, peak plasma concentrations occur in 4 to 5 hours. The plasma half-life of nicotinic acid is about one hour. In one study, fasting plasma concentrations were reported to be less than 20 ng/mL. In another study, it was reported that the administration of a single 1000 mg extended-release tablet resulted in mean nicotinic acid concentrations of less than 50 ng/mL.
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Test Location
Quest Diagnostics, California USA
Test Version
20-Sep-2022
Interface / Setup
HL7 Interface Codes
| Order Code | Result Codes | Units |
|---|---|---|
| VITB3 | 62652NICOTINIC ACID | ng/mL |
| 62653NICOTINAMIDE | ng/mL |
Test Version
20-Sep-2022