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Catecholamines LC-MS/MS, Plasma

Test ID: CAT P

Test

Method

LC-MS/MS

Report Includes

Dopamine; Epinephrine; Norepinephrine

Specimens

Plasma (EDTA)

Test Location

University Health Network, 200 Elizabeth St, 11E-444, Toronto ON M5G 2C4

Test Version

19-Mar-2024

Specimen

Specimens

Plasma (EDTA)

Sample Volume

5.0 mL
split into two equal aliquots

Minimum Volume

2.0 mL
split into two equal aliquots

Patient Preparation

Patient must refrain from eating, using tobacco and drinking caffeinated beverages for at least 4 hours before the specimen is drawn. Patient must be in supine position for at least 30 minutes prior to specimen collection.

Collection & Handling

Collection Instructions

Patient must be in supine position for at least 30 minutes prior to specimen collection. Place tube on ice immediately after collection.

Handling Information

Specimen should be kept cold and spun in a cold centrifuge as soon as possible, within 60 minutes. Split plasma into two equal aliquots and freeze immediately. Store and send frozen.

Stability

Frozen 1 week

Additional Stability Information

If the specimen thaws, it is unsuitable for analysis. Stable for 6 months at -70°C

Rejection Criteria

Specimen Not frozen

Test Version

19-Mar-2024

Performance / Interpretation

Method

LC-MS/MS

Turnaround Time

15 days

Results

Name Units Reference Range Conversion Factor
Norepinephrine nmol/L
  • Supine: 0.5 - 3.1
pg/mL x 0.00591
Epinephrine nmol/L
  • Supine: <1.1
pg/mL x 0.00546
Dopamine nmol/L
  • Supine: <0.13
Note: Higher catecholamine concentrations are observed in specimens collected from upright or standing adults.

Test Location

University Health Network, 200 Elizabeth St, 11E-444, Toronto ON M5G 2C4

Test Version

19-Mar-2024

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
CAT P 7065Norephinephrine (plasma) nmol/L
7067Epinephrine (plasma) nmol/L
63939Dopamine (plasma) nmol/L
40002Test Comment

Test Version

19-Mar-2024