Test Overview
Clinical Utility
To detect the presence of auto antibody specific to Desmoglein 1 and/or 3 in a patients serum as an aid to diagnose type of pemphigus
Method
Enzyme-linked immunosorbent assay (ELISA)
Result Included
Desmoglein 1 Antibodies; Desmoglein 3 Antibodies
Aliases/Synonyms
DSG 3; DSG 1; Pemphigus Foliaceus; Pemphigus Vulgaris
Specimen
Specimen Type
SerumContainers
Collection Containers
Gold top (SST)
Volume
Sample Volume
0.3 mL
Minimum Volume
0.1 mL
Patient Preparation
Fasting preferred
Collection & Handling
Collection Instructions
Blood should be collected fasting or at least one hour after meals to avoid lipemic serum
Handling Information
Allow the blood to clot at room temperature (20 to 25°C) and separate as soon as possible to limit hemolysis.
Stability
| Ambient | Refrigerated | Frozen |
|---|---|---|
| Unacceptable | 7 days | 28 days |
Rejection Criteria
| Criteria | Specification |
|---|---|
| Hemolysis | Gross |
| Lipemia | Gross |
| Other | Icteric, microbial growth |
| Specimen | Heat inactivated or not frozen |
Performance & Interpretation
Turnaround Time
11 days
Results
Name
Units
Reference Range
Conversion
-
Desmoglein 1 AntibodiesU/mLNegative: <14
Equivocal: 14 - 20
Positive: >20 -
Desmoglein 3 AntibodiesU/mLNegative: <9
Equivocal: 9 - 20
Positive: >20
Referral Location
Out-of-Country
Interface & Setup
HL7 Interface Codes
| Order Code | Result Name | Result Codes | Units |
|---|---|---|---|
| DESM13 S | DESMOGLEIN 1 ANTIBODY | 63346 | U/mL |
| DESMOGLEIN 3 ANTIBODY | 63347 | U/mL |
Test Version
Last Updated
2023-03-01