Share to
  • Email
  • Facebook
  • X
  • Bluesky
  • Instagram
  • LinkedIn
Share to
  • Email
  • Facebook
  • X
  • Bluesky
  • Instagram
  • LinkedIn
Back to Test Catalogue
Test ID: SC5B9 P

Soluble C5b-9 Complement, Plasma

Test Overview

Clinical Utility

sC5b-9 is the non-cytolytic, soluble form of the terminal complement complex C5b-9 (TCC or membrane attack complex MAC). The quantitative measurement of plasma level of sC5b-9 provides information on the degree of activation of the terminal complement pathway.
sC5b-9 testing can help to confirm the diagnosis of complement-related diseases1, 2 (aHUS or C3G for example). sC5b-9 testing can also help to monitor anti-complement therapy efficiency (Eculizumab)3, 4. SC5b-9 testing should be interpreted in the context of other clinical and laboratory evidence of disease activity.
1. Bu et al. http://dx.doi.org/10.1053/j.ajkd.2015.02.326
2. Cataland et al. Blood. 2014 Jun 12;123(24):3733-8
3. Wehling et al. Clinical and Experimental Immunology, 187: 304-315
4. Jodele et al. BBMT, 2016:22,307

Method

Enzyme-linked immunosorbent assay (ELISA)

Analytical Platform

MicroVue SC5b-9 Plus Enzyme Immunoassay (QUIDEL)

Result Included

Soluble C5b-9 Complement

Aliases/Synonyms

Soluble membrane attack complex (sMAC); soluble Terminal complement complex (sTCC)

Specimen

Specimen Type

Plasma

Containers

Preferred Containers

Plasma (EDTA lavender top)

Acceptable Containers

Plasma (Citrate light blue top)

Volume

Sample Volume

0.6 mL x 2

Minimum Volume

0.6 mL

Collection & Handling

Handling Information

Spin to separate immediately. Submit two aliquots if possible, each over 0.5 mL. Sample processing and storage must be completed within 2 hours. Store and send frozen.

Stability

Ambient Refrigerated Frozen
4 hours 4 hours >3 months at -70°C

If the specimen thaws, it is unsuitable for analysis.

Rejection Criteria

Criteria Specification
Specimen Not frozen at -70°C

Performance & Interpretation

Turnaround Time

16 days

Results

Name
Units
Reference Range
Conversion
  1. Soluble C5b-9 Complement
    ng/mL
    <300

    Comment

    The sC5B-9 complex is considered a surrogate marker of terminal complement activation by all complement pathways.

    Elevated concentrations of C5b-9 are associated with the development of complement-associated thrombotic microangiopathy (CA-TMA).

Referral Location

Canada

Interface & Setup

HL7 Interface Codes

Order Code Result Name Result Codes Units
SC5B9 P SC5b-9 63337 ng/mL

Test Version

Last Updated

2024-08-22