Thiamine diphosphate; Thiamine Pyrophosphate
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Vitamin B1, Whole Blood
Test ID: VITB1 WBLON
Test
Aliases/Synonyms
Method
LC-MS/MS
Report Includes
Vitamin B1
Specimens
Whole Blood (EDTA)
Clinical Utility
This assay measures the primary active form of Vitamin B1 (Thiamine diphosphate) and is the recommended test for assessment of body stores (thiamine deficiency). Thiamine and thiamine monophosphate are not measured.
Test Location
London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5
Test Version
18-Jan-2024
Specimen
Specimens
Whole Blood (EDTA)
Collection Containers
Lavender top (EDTA)
Sample Volume
2 mL
Minimum Volume
1 mL
Patient Preparation
Fasting (12 – 14 h) recommended but non-fasting is acceptable. Avoid vitamin supplementation for 24 hours prior to collection.
Collection & Handling
Handling Information
Immediately transfer to amber vial to protect from light and freeze.
Store and send frozen.
Additional Information
Requisition must indicate Whole Blood.
Stability
| Frozen | 28 days |
|---|
Additional Stability Information
If the specimen thaws, it is unsuitable for analysis.
Rejection Criteria
| Specimen | Not frozen or not protected from light |
|---|
Test Version
18-Jan-2024
Performance / Interpretation
Method
LC-MS/MS
Turnaround Time
8 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Vitamin B1 | nmol/L |
|
µg/dL x 29.6 | |
Test Location
London Health Sciences Centre, 339 Windermere Road, London ON N6A 5A5
Test Version
18-Jan-2024
Interface / Setup
Test Version
18-Jan-2024