PINP I
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Procollagen Type I Intact N-Terminal Propeptide, Serum
Test ID: PINP
Test
Aliases/Synonyms
Method
RIA (Radioimmunoassay)
Method Description
A known amount of labelled PINP and an unknown amount of unlabelled PINP in the sample compete for a limited number of high affinity binding sites on the polyclonal rabbit anti-PINP antibody. A second antibody, directed against rabbit IgG and coated to kaolin particles, is used to separate the antibody-bound PINP from free PINP. The radioactivity of the bound tracer antigen is measured in a gamma counter. The amount of labelled PINP in the sample tube is inversely proportional to the amount of PINP in the sample. The concentrations in unknown samples are obtained from a calibration curve, which is based on the concurrent testing of the UniQ PINP RIA calibrators that contain 0 to 250 µg/L PINP.
Platform
Orion Diagnostica UniQ PINP
Report Includes
Procollagen I Intact N-Terminal
Specimens
Serum
Clinical Utility
Intact N-terminal propeptide of type I procollagen is useful in the management of osteoporosis and monitoring bone formation therapies and antiresorptive therapies.
Test Location
Quest Diagnostics, California USA
Test Version
21-Sep-2023
Specimen
Specimens
Serum
Sample Volume
1.0 mL
Minimum Volume
0.3 mL
Stability
| Ambient | Unacceptable |
|---|---|
| Refrigerated | 27 days |
| Frozen | 60 days |
Additional Stability Information
Avoid repeated freezing and thawing.
Test Version
21-Sep-2023
Performance / Interpretation
Method
RIA (Radioimmunoassay)
Method Description
A known amount of labelled PINP and an unknown amount of unlabelled PINP in the sample compete for a limited number of high affinity binding sites on the polyclonal rabbit anti-PINP antibody. A second antibody, directed against rabbit IgG and coated to kaolin particles, is used to separate the antibody-bound PINP from free PINP. The radioactivity of the bound tracer antigen is measured in a gamma counter. The amount of labelled PINP in the sample tube is inversely proportional to the amount of PINP in the sample. The concentrations in unknown samples are obtained from a calibration curve, which is based on the concurrent testing of the UniQ PINP RIA calibrators that contain 0 to 250 µg/L PINP.
Platform
Orion Diagnostica UniQ PINP
Turnaround Time
8 days
Results
| Name | Units | Reference Range | Conversion Factor | |
|---|---|---|---|---|
| Procollagen I Intact N-Terminal | mcg/L |
|
||
Test Location
Quest Diagnostics, California USA
Test Version
21-Sep-2023
Interface / Setup
Test Version
21-Sep-2023