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Porphyrins Quantitation, 24 hr Urine

Test ID: PORPHQ U

Test

Method

HPLC

Report Includes

Uroporphyrin III; Heptacarboxylic Acid; Hexacarboxylic Acid; Pentacarboxylic; Coproporphyrin I; Coproporphyrin III; Copro III/Copro I; Interpretation

Specimens

Urine (24 hr)

Clinical Utility

Quantitation may only be done if a preliminary screen (billed separately) is positive.

Test Location

The Ottawa Hospital General Campus, 501 Smyth Road, Ottawa ON K1H 8L6

Test Version

10-Feb-2023

Specimen

Specimens

Urine (24 hr)

Sample Volume

20 mL

Specimen Comment

This collection is not suitable for ALA/PBG testing.

Collection & Handling

Collection Instructions

Collect 24 hour urine with 5 g sodium carbonate. Protect from light – collect in dark bottle. Keep cool during collection.

Handling Information

Final pH should be 7 to 10. Indicate volume (in litres) and collection date. Store and send frozen.

Test Version

10-Feb-2023

Performance / Interpretation

Method

HPLC

Turnaround Time

8 days

Results

Name Units Reference Range Conversion Factor
Uroporphyrin III - U (24 hr) nmol/d
  • Adult: ≤20
µg/24 hr x 1.20
Heptacarboxylic Acid nmol/d
  • Adult: ≤16
Hexacarboxylic Acid nmol/d
  • Adult: ≤2
Pentacarboxylic nmol/d
  • ≤2
Coproporphyrin I nmol/d
  • Adult: 5.0 - 90.0
µg/24 hr x 1.53
Coproporphyrin III nmol/d
  • Adult: 15 - 242
µg/24 hr x 1.53
Copro III/Copro I Ratio
  • Adult: 2.6 - 5.3
Porphyrin reference values are not available for individuals 17 y and younger.

Test Location

The Ottawa Hospital General Campus, 501 Smyth Road, Ottawa ON K1H 8L6

Test Version

10-Feb-2023

Interface / Setup

HL7 Interface Codes

Order Code Result Codes Units
PORPHQ U 20263Uroporphyrin III 24h Urine nmol/d
20264Heptacarboxylic Acid Porph 24h Urine nmol/d
20265Hexacarboxylic Acid Porph 24h Urine nmol/d
20266Pentacarboxylic Acid Porph 24h Urine nmol/d
20267Coproporphyrin I 24h Urine nmol/d
20268Coproporphyrin III 24h Urine nmol/d
20269Copro III:Copro I 24h Ratio
20270Interpretation

Test Version

10-Feb-2023