Test Overview
Clinical Utility
Diagnosis and follow-up of glucagonomas and other glucagon-producing tumors. Assessing diabetic patients with problematic hyper- or hypoglycemic episodes (extremely limited utility). Further information can be found on the performing laboratory’s website.
Method
Enzyme-linked immunosorbent assay (ELISA)
Method Description
The glucagon enzyme-linked immunosorbent assay (ELISA) is a quantitative two-step sandwich type immunoassay. In the first step, calibrators, controls, and unknown samples are added to glucagon antibody-coated microtiter wells and incubated with biotinylated glucagon antibody. After the first incubation and washing, the wells are incubated with streptavidin horseradish peroxidase conjugate (SHRP). After the second incubation and washing step, the wells are incubated with substrate solution (tetramethylbenzidine: TMB). After TMB incubation, an acidic stopping solution is added. In principle, the antibody-biotin conjugate binds to the solid phase antibody-antigen complex, which in turn binds to the streptavidin-enzyme conjugate. The antibody-antigen-biotin conjugate-SHRP complex bound to the well is detected by enzyme-substrate reaction. The degree of enzymatic turnover of the substrate is determined by dual wavelength absorbance measurement at 450 nm as primary test filter and 630 nm as reference filter. The absorbance measured is directly proportional to the concentration of glucagon in the samples and calibrators.(Package insert: Glucagon ELISA IFU. Ansh Labs, Revision 09, 01/2023)
Result Included
Glucagon
Specimen
Specimen Type
PlasmaVolume
Sample Volume
2.0 mL
Minimum Volume
0.45 mL
Patient Preparation
Patient should fast for 8 hours before specimen collection.
Collection & Handling
Collection Instructions
Pre-chill lavender top (EDTA) tube at 4°C before drawing the specimen. Draw blood into the pre-chilled tube and then process.
Handling Information
Pre-chill lavender top (EDTA) tube at 4°C before drawing the specimen. Draw blood into the pre-chilled tube and process as follows: Chill filled tube in wet ice for 10 minutes. Centrifuge in a refrigerated centrifuge or in a pre-chilled centrifuge carrier. Immediately after centrifugation, aliquot plasma into a plastic vial and freeze
Stability
| Frozen |
|---|
| 90 days |
Rejection Criteria
| Criteria | Specification |
|---|---|
| Hemolysis | Gross |
| Icterus | Gross |
| Lipemia | Gross |
Performance & Interpretation
Turnaround Time
11 days
Specimen Retention Time
14 days
Results
-
Glucagonpg/mL≥1 year of age: ≤159 pg/mL
<1 year of age: No reference interval establishedng/L x 1
Referral Location
Out-of-Country
Test Version
Last Updated
2026-01-21