Cytokine Panel 2 (TNFA, CXCL9, IFNG), Plasma
Test Overview
Method
Fluorescence immunoassay (FIA)
Analytical Platform
Proteinsimple ELLA<br>This analysis is performed at Hospital for Sick Children, Toronto, ON, CA
Result Included
Tumour Necrosis Factor Alpha (TNF-Alpha); CXCL 9/MIG; Interferon Gamma (IFN-G);
The three tests included in this profile may be ordered individually.
The price for the three-test profile is less than the combined price of any two of these tests ordered individually.
Tumour Necrosis Factor Alpha (TNF-Alpha), Plasma
CXCL 9/MIG, Plasma
Interferon Gamma (IFN-G), Plasma
Aliases/Synonyms
TNFa; MIG1; Monokine Induced by Gamma Interferon; IFNg; Interferon gamma
Specimen
Specimen Type
Plasma (EDTA)Containers
Collection Containers
Lavender top (EDTA)
Volume
Sample Volume
0.5 mL x 2
Minimum Volume
0.25 mL x 2
Collection & Handling
Handling Information
Separate within 30 minutes of collection. Centrifuge for 15 minutes at 1000 RCF. Prepare two 0.5 mL aliquots (min 2 x 0.25 mL). Store and ship frozen.
Additional Information
Avoid freeze-thaw cycles
Stability
| Ambient |
|---|
| Unacceptable |
Avoid freeze-thaw cycles
Rejection Criteria
| Criteria | Specification |
|---|---|
| Hemolysis | Gross |
| Lipemia | Gross |
Performance & Interpretation
Turnaround Time
14 days
Results
-
TNF-Alphapg/mL≤9.8
-
CXCL 9/MIGpg/mL≤657
-
IFN-Gammapg/mL≤3.3
Comment
Results should be interpreted in the context of the clinical presentation, therapeutic intervention, and additional laboratory data. Assay performance has not been validated in the context of treatment monitoring following administration of cytokine-blocking therapies; interpret results in this context with caution.
Referral Location
Canada
Interface & Setup
HL7 Interface Codes
| Order Code | Result Name | Result Codes | Units |
|---|---|---|---|
| CYTOTWO P | TNF-a | 64153 | pg/mL |
| CXCL9 | 64154 | pg/mL | |
| IFN-g | 64155 | ||
| Cytokine 2 Comment | 64443 |
Test Version
Last Updated
2022-06-23