Test Overview
Method
Chromatography/Mass Spectrometry
Result Included
Nicotinamide; Nicotinic Acid
Aliases/Synonyms
Nicotinamide; Nicotinic Acid; Niacin
Specimen
Specimen Type
Gel-separator tubes are not acceptableSerum
Plasma
Containers
Preferred Containers
Red top (No additive)
Acceptable Containers
Lavender top (EDTA)
Volume
Sample Volume
1.0 mL
Minimum Volume
0.5 mL
Specimen Comment
Avoid gel-separator tubes.
Collection & Handling
Handling Information
Store and submit specimen protected from light in an amber vial.
Store and send frozen.
Stability
| Ambient | Refrigerated | Frozen |
|---|---|---|
| Unacceptable | Unacceptable | 30 days |
Rejection Criteria
| Criteria | Specification |
|---|---|
| Specimen | Gel tube or not protected from light |
Performance & Interpretation
Turnaround Time
7 days
Results
-
Nicotinamideng/mL
Comment
Nicotinamide is a metabolite of nicotinic acid. Due to the large variability in the metabolism of nicotinic acid, plasma concentrations of this metabolite are variable. In one study, fasting plasma concentrations were reported to be approximately 40 ng/mL. In another study it was reported that the administration of a single 1000 mg of extended-release tablet of nicotinic acid resulted in a mean peak nicotinamide concentration of 400 ng/mL between 5 and 10 hours post dose, decreasing to about 100 ng/mL by 16 hours post dose.
-
Nicotinic Acidng/mL
Comment
Due to the large variability in the metabolism of nicotinic acid, the dosing preparation used (immediate-release vs. extended release), and the mg doses used, the serum concentrations may range from less than 20 ng/mL to about 30,000 ng/mL. After oral administration of an immediate-release tablet, peak plasma concentrations occur in 4 to 5 hours. The plasma half-life of nicotinic acid is about one hour. In one study, fasting plasma concentrations were reported to be less than 20 ng/mL. In another study, it was reported that the administration of a single 1000 mg extended-release tablet resulted in mean nicotinic acid concentrations of less than 50 ng/mL.
Referral Location
Out-of-Country
Interface & Setup
HL7 Interface Codes
| Order Code | Result Name | Result Codes | Units |
|---|---|---|---|
| VITB3 | NICOTINIC ACID | 62652 | ng/mL |
| NICOTINAMIDE | 62653 | ng/mL |
Test Version
Last Updated
2022-09-20