Collapsin Response-Mediator Protein-5-IgG (CRMP-5-IgG) Western Blot, CSF
Test Overview
Clinical Utility
This testing is recommended in cases of chorea, vision loss, cranial neuropathy and myelopathy.
Clinical Information at external website.
Method
Western Blot
Method Description
Western blot is performed on denatured full-length recombinant humancollapsin response-mediator protein-5 (CRMP-5), reduced and subjected to electrophoresis on 10% polyacrylamide gel. IgG is detected autoradiographically by enhanced chemiluminescence.(Yu Z, Kryzer TJ, Griesmann GE, et al: CRMP-5 neuronal autoantibody: marker of lung cancer and thymoma-related autoimmunity. Ann Neurol 2001 February;49[2]:146-154)
Result Included
CRMP-5-IgG Western Blot
Specimen
Specimen Type
CSFVolume
Sample Volume
3 mL
Minimum Volume
2 mL
Collection & Handling
Handling Information
Store and send frozen
Stability
| Ambient | Refrigerated | Frozen |
|---|---|---|
| 72 hours | 28 days | 28 days |
Rejection Criteria
| Criteria | Specification |
|---|---|
| Hemolysis | Gross |
| Lipemia | Gross |
| Other | Grossly icteric |
Performance & Interpretation
Turnaround Time
10 days
Results
-
CRMP-5-IgG Western BlotNoneNegative
Comment
References:
Yu Z, Kryzer TJ, Griesmann GE, et al: CRMP-5 neuronal autoantibody: marker of lung cancer and thymoma-related autoimmunity. Ann Neurol 2001 February;49(2):146?154.
Cross SA, Salomao DR, Parisi JE, et al: Paraneoplastic autoimmune optic neuritis with retinitis defined by CRMP-5-IgG. Ann Neurol 2003;54:38?50
Referral Location
Out-of-Country
Interface & Setup
HL7 Interface Codes
| Order Code | Result Name | Result Codes | Units |
|---|---|---|---|
| CRMP CSF | CRMP-5-IgG Western Blot, CSF | 63136 | None |
Test Version
Last Updated
2019-12-09