Procollagen Type I Intact N-Terminal Propeptide, Serum
Test Overview
Clinical Utility
Intact N-terminal propeptide of type I procollagen is useful in the management of osteoporosis and monitoring bone formation therapies and antiresorptive therapies.
Method
Radioimmunoassay (RIA)
Analytical Platform
Orion Diagnostica UniQ PINP
Method Description
A known amount of labelled PINP and an unknown amount of unlabelled PINP in the sample compete for a limited number of high affinity binding sites on the polyclonal rabbit anti-PINP antibody. A second antibody, directed against rabbit IgG and coated to kaolin particles, is used to separate the antibody-bound PINP from free PINP. The radioactivity of the bound tracer antigen is measured in a gamma counter. The amount of labelled PINP in the sample tube is inversely proportional to the amount of PINP in the sample. The concentrations in unknown samples are obtained from a calibration curve, which is based on the concurrent testing of the UniQ PINP RIA calibrators that contain 0 to 250 µg/L PINP.
Result Included
Procollagen I Intact N-Terminal
Aliases/Synonyms
PINP I
Specimen
Specimen Type
SerumVolume
Sample Volume
1.0 mL
Minimum Volume
0.3 mL
Stability
| Ambient | Refrigerated | Frozen |
|---|---|---|
| Unacceptable | 27 days | 60 days |
Avoid repeated freezing and thawing.
Performance & Interpretation
Turnaround Time
8 days
Results
-
Procollagen I Intact N-Terminalmcg/LMales:
<23 y: Not established
23 - 60 y: 30-110
≥60 y: Not established
Females:
<20 y: Not established
20 - 45 y: 22 - 104
46-60y: 20-108
≥60 y: Not established
Referral Location
Out-of-Country
Test Version
Last Updated
2023-09-21